【摘 要】
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Objective:To examine the effect of metanol extract of Petiveria alliacea (PM) on airway inflammation in a murine model of chronic asthma.Methods:Two-month-old male BALB/c mice (n=6--8/group) were sensitized on days 0 and 14 by intraperitoneal injection of
【机 构】
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Natural Products Research Laboratory, Higher School of Chemical Engineering and Extractive Industrie
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Objective:To examine the effect of metanol extract of Petiveria alliacea (PM) on airway inflammation in a murine model of chronic asthma.Methods:Two-month-old male BALB/c mice (n=6--8/group) were sensitized on days 0 and 14 by intraperitoneal injection of 20 μ g ovalbumin (OVA).On day 25,the mice received an airway challenge with OVA (3％,w/v,in phosphate buffered saline).PM was administered orally by oral gavage to mice at doses of 100,200 and 400 mg/kg body weight once daily from days 18 to 23.Control mice were orally administered phosphate buffered saline (PBS) to induce a model of asthma.At the end of the test,respiratory reactivity was assayed,the total cell number,interleukin-4 (IL-4),IL-5,IL-13,tumor necrosis factor-alpha (TNF-α) and reactive oxygen species (ROS) in the bronchoalveolar lavage fluid (BALF) were determined and the levels of serum IgE,intercellular cell adhesion molecule 1 (ICAM-1) and eotoxin were measured.In addition,lung tissue was used to qualify the IL-4,IL-5,IL-13,TNF-α and transforming growth factor beta 1 (TGF-β 1).Histologic examination was performed to observe inflammatory cellular infiltration.Results:The administration of PM in comparison with the OVA-only treated group significantly attenuated the infiltration of eosinophils and other inflammatory cells (P＜0.01).Airway resistance (RI) in the OVA-only induced group was significantly higher than that of the PBS control group (P＜0.01) when methacholine was added.TNF-α,IgE,TGF-β 1 and cytokine levels IL-4,IL-5,IL-13 in the BALF decreased compared to control mice (P＜0.01 or P＜0.05).PM treatment also inhibited the production of chemokines,eotaxin and ICAM-1 in BALF (P＜0.01),which improved lung function.Histopathological examination revealed that the sensitized treated PM groups had significant lower in inflammatory scores similar to dexamethasone treatments and the untreated group.Conclusion:Administration of PM could inhibit airway inflammation,regulate cytolines,chemokines and enhance pulmonary conditions in allergic murine model of asthma.
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