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目的建立实验性自身免疫性脑脊髓炎（ＥＡＥ）动物模型，探讨口服自身抗原诱导免疫耐受对大鼠ＥＡＥ的防治作用。方法在普通Ｗｉｓｔａｒ大鼠经１次足跖真皮内注射完全弗氏佐剂－豚鼠全脊髓匀浆或完全弗氏佐剂－髓鞘碱性蛋白乳剂加百日咳疫苗诱发ＥＡＥ疾病；另在大鼠致炎前及发病后口服髓鞘碱性蛋白（ＭＢＰ），观察其对ＥＡＥ的防治作用。结果在Ｗｉｓｔａｒ大鼠成功地诱发了ＥＡＥ，发病率将近９０％。大鼠在致炎前口服ＭＢＰ，可明显推迟ＥＡＥ发病时间，降低ＥＡＥ发病率，使神经组织病理改变明显改善。大鼠发生ＥＡＥ后给予ＭＢＰ，可明显控制发病动物的病情，使病程缩短及减轻患鼠神经组织的炎症程度。另外，在耐受鼠由ＭＢＰ引起的迟发型超敏反应（ＤＴＨ）以及体外针对ＭＢＰ的淋巴细胞增殖反应也明显受到抑制。结论口服ＭＢＰ可引起特异性的免疫耐受，从而产生对实验性自身免疫性脑脊髓炎的防治作用
Objective To establish an animal model of experimental autoimmune encephalomyelitis (EAE) and investigate the preventive and therapeutic effects of oral autoantigen on immune tolerance induced by EAE in rats. Methods Wistar rats were injected with complete Freund’s adjuvant-guinea pig whole spinal cord homogenate or Freund’s complete Freund’s adjuvant-myelin basic protein emulsion plus pertussis vaccine in dermal infusions of normal Wistar rats. EAE disease was induced in rats Pre and post-inflammatory oral myelin basic protein (MBP), to observe the prevention and treatment of EAE. The results successfully induced EAE in Wistar rats, the incidence rate of nearly 90%. Oral administration of MBP to rats before inflammation can delay the onset of EAE, reduce the incidence of EAE, and significantly improve the pathological changes of nerve tissue. After the occurrence of EAE rats given MBP, can significantly control the disease in animals, shorten the course of the disease and reduce the degree of inflammation in rats with nerve tissue. In addition, lymphocyte proliferation responses against MBP-induced delayed-type hypersensitivity (DTH) in mice and MBP in vitro were also significantly inhibited. Conclusion oral MBP can cause specific immune tolerance, resulting in prevention and treatment of experimental autoimmune encephalomyelitis