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【目的】应用BOX-PCR技术对12株南苜蓿(Medicago polymorpha)接种根瘤菌菌株在土壤中的竞争结瘤能力进行研究,以占瘤率的高低配合植物生长性状最终确定与南苜蓿共生的高效根瘤菌菌株。【方法】对接种根瘤菌产生的南苜蓿根瘤进行分离培养,选择部分结瘤菌株和12株全部供试接种菌株进行BOX分子指纹图谱研究,经过对图谱的比较与分析获得了各接种菌株的占瘤率。【结果】在供试菌株中,来自于云南盈江的菌株SWF67523占瘤率最高,为93.33%,说明菌株SWF67523具有较强的竞争结瘤能力,该菌株对南苜蓿干重增幅也较高,为100%;菌株SWF67409来自于云南楚雄,其占瘤率略低于菌株SWF67523,但其对提高植物干重的贡献最大(增幅106.5%);来自于云南楚雄的菌株SWF67394占瘤率较低,但其结瘤率高,对植物生长的影响作用也较明显。【结论】将根瘤菌菌株的竞争结瘤能力纳入南苜蓿高效根瘤菌菌株的筛选中,研究获得了一株竞争结瘤能力强、显著提高植物生物量的菌株SWF67523,以及促生根瘤菌菌株SWF67409和SWF67394,为生产高效根瘤菌菌剂提供了物质基础。
【Objective】 Competent nodulation ability of 12 strains of Rhizobia inoculated with Medicago polymorpha in soil was studied by using BOX-PCR technique, and the high rate Rhizobium strains. 【Method】 The root nodules of S. alfalfa produced by inoculation with Rhizobia were isolated and cultured. The partial nodulation strains and 12 strains of inoculated strains were selected for the study of molecular fingerprinting of BOX. After comparing and analyzing the patterns, Tumor rate. 【Result】 Among the tested strains, strain SWF67523 from Yingjiang, Yunnan accounted for the highest rate of 93.33%, indicating that strain SWF67523 had a strong competitive nodulation ability. The dry weight of S. alfalfa increased significantly, Was 100%. The strain SWF67409 was from Chuxiong, Yunnan, accounting for slightly less than the strain SWF67523, but its contribution to increasing plant dry weight was the largest (106.5% increase). The strain SWF67394 from Chuxiong, Yunnan Province, However, its high nodulation rate, the impact of plant growth is also more obvious. 【Conclusion】 The competitive nodulation ability of Rhizobia strains was included in the screening of the high efficient rhizobia strains of S. alfalfa. A strain SWF67523 with strong ability of nodulation and significant increase of plant biomass was obtained, as well as strain SWF67409 And SWF67394, provide the material basis for the production of efficient rhizobia agents.