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目的:检测HBeAg阴性慢性乙型肝炎抗病毒治疗患者血清中乙型肝炎病毒外膜大分子表面抗原蛋白(Hepatitis B Virus Large Surface Protein,LHBs)、前S1抗原(PreS1 Ag)、丙氨酸氨基转移酶(ALT)和HBV DNA水平,探讨HBV DNA与LHBs、PreS1 Ag、ALT的关系,LHBs水平对HBV复制状态的判定和对临床抗病毒治疗的指导意义。方法:对60例接受抗病毒治疗的HBeAg阴性慢性乙型肝炎患者,定期采集患者血清标本,采用酶联免疫吸附试验(ELISA)法检测LHBs和PreS1 Ag,速率法测定ALT,用实时荧光定量PCR法检测HBV DNA。结果:抗病毒治疗0、9、12个月,LHBs阳性率与HBV DNA阳性率差异无统计学意义(P>0.05),均显著高于PreS1 Ag阳性率;LHBs含量与HBV DNA拷贝数对数呈正相关性,相关系数r=0.825;LHBs OD值和HBV DNA拷贝数随抗病毒治疗时间的延长同步下降。结论:血清LHBs是一个操作简便的能反映体内HBV复制水平,抗病毒治疗过程中和HBV DNA同步下降,可用于判断HBV复制程度和用于指导抗病毒治疗的敏感指标。
Objective: To detect the levels of serum hepatitis B virus surface protein (LHBs), preS1 antigen (PreS1 Ag), alanine aminotransferase Enzyme (ALT) and HBV DNA levels, to explore the relationship between HBV DNA and LHBs, PreS1 Ag, ALT, LHBs level of HBV replication state and its clinical significance of anti-viral treatment. Methods: Serum samples were collected from 60 HBeAg-negative chronic hepatitis B patients receiving antiviral therapy. LHBs and PreS1 Ag were detected by enzyme-linked immunosorbent assay (ELISA) and ALT by rate method. Real-time fluorescence quantitative PCR Method for detection of HBV DNA. Results: There was no significant difference in the positive rate of LHBs and the positive rate of HBV DNA at 0, 9, and 12 months after antiviral therapy (all P> 0.05), which were significantly higher than those of PreS1 Ag. The LHBs and HBV DNA copy number logarithm The correlation coefficient r = 0.825; LHBs OD value and HBV DNA copy number decreased with the prolongation of antiviral treatment time. Conclusions: Serum LHBs is a sensitive indicator that can reflect the level of HBV replication in vivo and decrease synchronously with HBV DNA during anti-virus therapy. It can be used as a sensitive index to judge the degree of HBV replication and to guide anti-virus therapy.