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目的:探讨IL-10基因启动子区-592A/C多态性与河北省唐山地区汉族妇女HPV感染相关宫颈癌发生的相关性。方法:应用PCR、PCR-RFLP技术对103例汉族妇女宫颈癌患者和115例汉族妇女正常宫颈组织的IL-10基因启动子区域-592多态性及HR-HPV16/18的感染进行检测分析。结果:IL-10基因启动子区-592A/C多态性3种基因型CC、CA、AA在唐山地区汉族妇女宫颈癌中所占比例差异无统计学意义(2χ=5.942,P=0.051);HR-HPV16/18 DNA检测结果显示,HPV16、18感染在宫颈癌及对照组中差异有统计学意义(HPV16:2χ=63.455,P=0.000;HPV18:2χ=17.423,P=0.000),IL-10基因启动子区-592A/C多态性AA、CA、CC3种基因型在HPV16、18检测阳性组和阴性组中差异有统计学意义,AA基因型是HPV16感染的易感基因型,增加了HPV16感染的风险性;CA及AA基因型增加了HPV18感染的易感性(PCA=0.043,RRCA=0.390,95%CI:0.134~1.138;PAA=0.035,RRAA=1.507,95%CI:1.107~2.053)。结论:在唐山地区汉族妇女IL-10基因启动子区-592A/C多态性可能与HPV16、18感染导致宫颈癌具有相关性。
Objective: To investigate the association between the -592A / C polymorphism of IL-10 gene promoter region and cervical cancer associated with HPV infection in Han women of Tangshan area in Hebei province. Methods: The -592 polymorphism of IL-10 gene promoter region and HR-HPV16 / 18 infection in 103 cervical cancer patients from Han women and 115 normal Han women were detected by PCR and PCR-RFLP. Results: There was no significant difference in the proportions of CC, CA and AA among the Han women with cervical cancer in Tangshan region (2χ = 5.942, P = 0.051) ; HR-HPV16 / 18 DNA test results showed that HPV16,18 infection in cervical cancer and control group, the difference was statistically significant (HPV16: 2χ = 63.455, P = 0.000; HPV18: 2χ = 17.423, P = 0.000) -10 gene promoter region -592A / C polymorphism AA, CA, CC genotypes in the HPV16,18 positive and negative detection group, the difference was statistically significant AA genotype HPV16 infection susceptibility genotypes, Increased the risk of HPV16 infection; CA and AA genotypes increased susceptibility to HPV18 infection (PCA = 0.043, RRCA = 0.390, 95% CI: 0.134-1.1338; PAA = 0.035, RRAA = 1.507, 95% CI: 1.107 ~ 2.053). Conclusion: The -592A / C polymorphism of IL-10 gene promoter in Han women in Tangshan area may be related to the HPV16,18 infection leading to cervical cancer.