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目的:探讨脂联素(APN)在高糖孵育血管内皮细胞中的表达调节及意义。方法:用不同浓度葡萄糖(5.5、11、22及33mM)孵育人脐静脉内皮细胞(HUVECs),2天后收集细胞和上清液。用实时荧光定量RT-PCR方法检测细胞中APN、肿瘤坏死因子α(TNFα)和过氧化物酶体增生物激活受体γ(PPARγ)的mRNA表达,用ELISA方法检测细胞上清液中APN和TNFα分泌情况。结果:随培养液中葡萄糖浓度的升高,HUVECs中APN的mRNA表达及上清中APN蛋白含量降低(p<0.05),TNFα的mRNA表达及上清中TNFα蛋白含量增加(p<0.05),HUVECs中PPARγ的mRNA表达降低(p<0.05),且组间比较差异均有统计学意义(p<0.05)。结论:高糖诱导血管内皮细胞保护性细胞因子APN表达分泌下降,可能是高糖诱导的血管内皮细胞功能损害的重要机制之一。
Objective: To investigate the regulation of adiponectin (APN) expression in vascular endothelial cells incubated with high glucose. Methods: Human umbilical vein endothelial cells (HUVECs) were incubated with different concentrations of glucose (5.5, 11, 22 and 33 mM) and cells and supernatants were collected after 2 days. The mRNA expressions of APN, TNFα and PPARγ in cells were detected by real-time fluorescence quantitative RT-PCR. The levels of APN, TNFα secretion. Results: The mRNA expression of APN and the content of APN in the supernatant of HUVECs were decreased (p <0.05), the mRNA expression of TNFα and the content of TNFα in the supernatant were increased with the increase of glucose concentration in the culture medium (p <0.05) The mRNA expression of PPARγ in HUVECs was decreased (p <0.05), and the difference between the two groups was statistically significant (p <0.05). CONCLUSION: High glucose can induce the decrease of APN expression and secretion in vascular endothelial cells, which may be one of the important mechanisms of high glucose-induced endothelial dysfunction.