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目的研究补中益气汤对人胃癌细胞株酶活性影响,探讨该药作用机制。方法以人胃腺癌细胞株SGC-7901为受试对象,利用血清药理学和酶细胞化学染色法,观察SGC-7901人胃腺癌细胞株的酶活性的改2+变。结果酶细胞化学法显示,中药组细胞质膜标志酶Mg-ATP酶和内质网标志酶G-6-P酶的酶反应颗粒小,数量减少,阳性细胞两种酶的表达量明显低于对照组(<0.05)。结论补中益气汤抗肿瘤作用可能与其降低2+胃癌细胞株Mg-ATP酶和G-6-P酶的活性相关。
Objective To study the effect of Buzhong Yiqi Decoction on the enzymatic activity of human gastric cancer cell lines and explore its mechanism. Methods SGC-7901 human gastric adenocarcinoma cell line SGC-7901 was chosen as the test object. Serum pharmacology and enzymatic cytochemical staining were used to observe the change of enzyme activity in SGC-7901 human gastric adenocarcinoma cell line. Results Enzyme-linked immunosorbent assay showed that the enzymatic reaction between the plasma membrane-bound enzyme Mg-ATPase and the endoplasmic reticulum enzyme G-6-P was decreased and the number of positive cells was significantly lower than that of the control Group (<0.05). Conclusion The anti-tumor effect of Buzhong Yiqi Decoction may be related to the decrease of the activities of Mg-ATPase and G-6-Pase in gastric cancer cell lines.2.