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目的:建立毛细管区带电泳(CZE)法测定黄精和玉竹多糖含量的方法,并用CZE法测定其单糖组成,为探讨这两种功效相近中药材的药效物质基础提供参考。方法:通过正交试验对黄精和玉竹多糖降解条件进行优化。以1-苯基-3-甲基-5-吡唑啉酮(PMP)为单糖的柱前衍生化试剂,用CZE法测定黄精和玉竹多糖的含量及其单糖组成。同时与采用苯酚-硫酸法测定黄精和玉竹多糖含量的结果进行比较。结果:黄精和玉竹多糖均由木糖、阿拉伯糖、葡萄糖、鼠李糖、甘露糖、半乳糖、葡萄糖醛酸、半乳糖醛酸等组成,但组成比例差异显著。结论:CZE法用于测定黄精和玉竹多糖的含量及单糖组成准确、可靠,结果满意。其单糖组成的异同一定程度上佐证了两者功效的相近和区别,提示两者临床上不能混淆使用。
Objective: To establish a capillary zone electrophoresis (CZE) method for the determination of Polygonatum odoratum and Polygonatum polysaccharide content, and CZE method to determine the monosaccharide composition, to explore these two similar efficacy of traditional Chinese medicine material basis for reference. Methods: The orthogonal test was used to optimize the degradation conditions of Polygonatum. The pre-column derivatization reagent with 1-phenyl-3-methyl-5-pyrazolone (PMP) as monosaccharide was used to determine the content and monosaccharide composition of polygonatum sibiricum and polygonatum polysaccharide with CZE method. At the same time with the phenol - sulfuric acid method for the determination of Polygonatum Polygonatum polysaccharides and the results were compared. Results: Polygonatum and polygonatum were composed of xylose, arabinose, glucose, rhamnose, mannose, galactose, glucuronic acid, galacturonic acid and other components, but the composition ratio was significantly different. Conclusion: CZE method is used to determine the content of polysaccharides from Polygonatum odoratum and Polygonatum, and the composition of monosaccharides is accurate and reliable. The results are satisfactory. The similarities and differences of their monosaccharide composition to a certain extent, corroborated the similarities and differences between the two effects, suggesting that the two can not be used clinically.