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利用腺病毒表达系统在肝癌细胞中成功地表达了有生物活性的白细胞介素－１２（ＩＬ－１２）。ＩＬ－１２的Ｐ３５和Ｐ４０ｃＤＮＡ分别克隆到腺病毒载体ｐＡＣＣＭＶｐＬｐＡ，构建ｐＡＣ／Ｐ３５和ｐＡＣ／Ｐ４０表达质粒。与腺病毒重组质粒ｐＪＭ１７共转染２９３细胞，通过基因重组产生ＩＬ－１２Ｐ３５和Ｐ４０重组腺病毒。用重组腺病毒感染肝癌细胞株ＨｅｐＧ２和ＳＭＭＣ７７２１，经ＥＬＩＳＡ和Ｗｅｓｔｅｒｎ检测证明，在这两株细胞中均有ＩＬ－１２的表达，并且经ＩＦＮ－γ诱生法检测表明，在ＨｅｐＧ２和ＳＭＭＣ７７２１细胞中表达的ＩＬ－１２具有诱导产生ＩＦＮ－γ的生物学活性。本实验为研究ＩＬ－１２对肝癌的基因治疗奠定了基础。
The bioactive interleukin-12 (IL-12) was successfully expressed in hepatoma cells using an adenovirus expression system. IL-12 P35 and P40 cDNAs were cloned into adenoviral vector pACCMVpLpA to construct pAC/P35 and pAC/P40 expression plasmids. 293 cells were co-transfected with adenovirus recombinant plasmid pJM17 and IL-12P35 and P40 recombinant adenoviruses were generated by gene recombination. Hepatoma cell lines HepG2 and SMMC7721 were infected with recombinant adenovirus. The expression of IL-12 in both cells was confirmed by ELISA and Western detection, and detected by IFN-γ induction method in HepG2 and SMMC7721 cells. The expressed IL-12 has the biological activity of inducing IFN-γ production. This experiment laid the foundation for the study of IL-12 gene therapy for liver cancer.