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本文构建了含SV40串联启动子的红细胞生成素（EPO）逆转录病毒表达载体。因逆转录病毒载体pN2A需用外源启动子才能表达目的的基因，故把SV40串联启动子及包括除信号肽中第2、3、4位氨基酸外的所有编码区及两个内含子序列的EPO基因片段一起克隆到pN2A载体neo基因下游的多聚接头上的单一酶切位点BglⅡ上，构建成重组质粒pN2ASV40EPO。
In this paper, an erythropoietin (EPO) retrovirus expression vector containing the SV40 tandem promoter was constructed. Since the retroviral vector pN2A requires the use of an exogenous promoter to express the gene of interest, the SV40 tandem promoter and all coding regions except for the amino acids 2, 3 and 4 of the signal peptide and two intronic sequences EPO gene fragment was cloned into a single restriction enzyme site BglII on the polylinker downstream of the neo gene of pN2A vector to construct a recombinant plasmid pN2ASV40EPO.