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目的探讨烫伤后不同组织脂多糖结合蛋白（ＬＢＰ）ｍＲＮＡ的动态表达及其与内毒素血症的关系。方法采用大鼠３５％体表面积Ⅲ度烫伤模型，实验动物随机分为正常对照组、烫伤组和多粘菌素Ｂ治疗组。血浆内毒素水平测定采用改良过氯酸预处理血浆、偶氮显色法鲎实验定量测定，组织ＬＢＰｍＲＮＡ含量采用ＲＴ－ＰＣＲ法。结果烫伤后门静脉及体循环内毒素水平均显著升高，８小时达峰值。给予多粘菌素Ｂ治疗可不同程度地降低血浆内毒素水平。正常组织可表达少量ＬＢＰ，烫伤后２小时不同组织ＬＢＰｍＲＮＡ表达较对照值有显著升高（Ｐ＜０．０５～０．０１），８小时达峰值，约为对照值的２～２．５倍，且一直持续至伤后２４小时。给予多粘菌素Ｂ抗内毒素治疗，可不同程度抑制肺、肠、肾组织ＬＢＰｍＲＮＡ的表达（Ｐ＜０．０５～０．０１）。相关分析显示，门静脉内毒素水平与肺组织ＬＢＰｍＲＮＡ呈显著正相关（ｒ＝０．４１２，Ｐ＜０．０５），但与肝、肠、肾组织无显著相关性。结论组织ＬＢＰｍＲＮＡ表达在烧伤后显著增多，肠源性内毒素血症可能是刺激组织ＬＢＰ基因表达的重要因素之一
Objective To investigate the dynamic expression of lipopolysaccharide binding protein (LBP) mRNA in different tissues after scald and its relationship with endotoxemia. Methods 35% body surface area of rats with a third degree scald model, the experimental animals were randomly divided into normal control group, scald group and polymyxin B treatment group. Plasma endotoxin levels were determined by modified perchloric acid pretreatment of plasma, azo coloration assay was used to determine the amount of LBP mRNA and the content of LBP mRNA was determined by RT-PCR. Results After scald, the levels of endotoxin and endotoxin in the systemic circulation increased significantly, reaching the peak at 8 hours. Administration of polymyxin B reduced plasma endotoxin levels to varying degrees. The expression of LBP mRNA in different tissues at 2h after scald was significantly increased compared with the control (P <0.05 ~ 0.01), and peaked at 8 hours, which was about 2 ~ 2.5 times of the control , And continued until 24 hours after injury. Given polymyxin B anti-endotoxin treatment, can inhibit the expression of LBP mRNA in lung, intestine and kidney tissue to varying degrees (P <0.05 ~ 0.01). Correlation analysis showed that the level of endotoxin in portal vein was positively correlated with LBP mRNA in lung tissue (r = 0.412, P <0.05), but not in liver, intestine and kidney. Conclusion The expression of LBP mRNA in the tissue increased significantly after burns, and intestinal endotoxemia may be one of the important factors to stimulate the expression of LBP gene