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目的研究胃癌组织中Krülppel样核转录因子-4(KLF4)和核转录因子特异性蛋白-1(Sp1)、Notch跨膜受体-1(Notch1)的表达,探讨KLF4和Sp1、Notch1在胃癌组织中表达的相关关系及三者与胃癌临床病理的关系。方法采用免疫组织化学染色法,分别对76例胃癌手术标本及30名同期收集的正常胃组织标本中Sp1、Notch1和KLF4进行蛋白检测,分析三者的表达水平与临床病理的关系及KLF4的表达与Sp1、Notch1表达的关系。结果正常胃组织标本中可见KLF4蛋白强阳性表达率为77%,在胃癌组织中表达较弱或表达缺失;Sp1在胃癌组织中阳性表达率为63%,正常组13%;Notch1在胃癌组织中阳性表达为66%,明显高于正常胃组织的23%,且三者表达与胃癌分化程度、胃癌分期及淋巴结转移密切相关(P<0.05)。KLF4和Notch1在胃癌中表达呈负相关(r=-0.629,P<0.05),KLF4和Sp1在胃癌组织中表达呈负相关(r=-0.566,P<0.05)。结论 KLF4和Sp1、Notch1在胃癌组织中表达失衡可能对胃癌的发生发展起重要作用,三者可能成为胃癌预后的判断指标及潜在的治疗靶点。
Objective To investigate the expression of KLF4, Sp1 and Notch1 in gastric cancer and to explore the role of KLF4, Sp1 and Notch1 in gastric cancer And the relationship between the three and the clinicopathological features of gastric cancer. Methods Immunohistochemical staining was used to detect the expression of Sp1, Notch1 and KLF4 in 76 specimens of gastric cancer and 30 specimens of normal gastric tissue collected from the same period respectively. The relationship between the expression of KLF4 and KLF4 was analyzed. And Sp1, Notch1 expression. Results The positive expression rate of KLF4 protein in normal gastric tissue specimens was 77%, while the expression of KLF4 protein in gastric cancer tissues was weak or the expression was low. The positive expression rate of Sp1 in gastric cancer tissues was 63% and 13% The positive expression was 66%, which was significantly higher than that of normal gastric tissue (23%). The expression of these three proteins was closely related to the differentiation degree of gastric cancer, gastric cancer staging and lymph node metastasis (P <0.05). The expression of KLF4 and Notch1 in gastric cancer was negatively correlated (r = -0.629, P <0.05). The expression of KLF4 and Sp1 in gastric cancer was negatively correlated (r = -0.566, P <0.05). Conclusion Unbalanced expression of KLF4, Sp1 and Notch1 in gastric cancer may play an important role in the occurrence and development of gastric cancer. The three may play an important role in the prognosis of gastric cancer and may be potential therapeutic targets.