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作者用PCR技术扩增恶性疟原虫裂殖子表面蛋白Ⅰ型和Ⅱ型(MSP-1和MSP-2)多态性基因,以鉴别生活在两类不同流行区的无症状疟原虫携带者的寄生虫特征。 血样采自塞内加尔Dielmo村的儿童和成人。昆虫学接种率为每人每周被感染蚊叮咬3—6次。3个月内每隔两周采血一次,以PCR测定。研究对象为厚血膜镜检疟原虫阳
The authors used PCR to amplify the genes encoding the type I and type II (MSP-1 and MSP-2) of Plasmodium falciparum merozoites to identify asymptomatic Plasmodium carriers living in two different endemic areas Parasite characteristics. Blood samples were collected from children and adults in Dielmo village, Senegal. Entomology vaccination rate per week per person infected mosquito bites 3-6 times. Blood samples were taken every two weeks for 3 months to be determined by PCR. The object of study was thick film microscopy of Plasmodium yang