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目的研究普仑司特对东莨菪碱诱导小鼠学习记忆损害的影响及其作用机制。方法采用连续腹腔注射东莨菪碱(0.8 mg·kg-1·d-1)诱导小鼠学习记忆损害模型,灌胃给予普仑司特(0.3,0.6,1.2 mg·kg-1·d-1),14 d后用Morris水迷宫和Y迷宫实验评价学习记忆功能,并测定脑内乙酰胆碱(Ach)、乙酰胆碱酯酶(Ach E)水平。结果普仑司特(0.3~1.2 mg·kg-1·d-1)能显著缩短小鼠在隐藏平台实验中寻找平台的潜伏期,增加空间探索实验中在原平台所在象限的停留时间和穿越原平台所在位置的次数,增加Y迷宫测试中正确反应次数。普仑司特还能显著增加小鼠脑组织海马区和皮层区Ach含量,并降低Ach E活性。结论普仑司特通过降低脑内Ach E活性,提高Ach含量,改善东莨菪碱诱导的小鼠学习记忆损害。
Objective To study the effect and mechanism of pranlukast on scopolamine-induced learning and memory impairment in mice. Methods The mice model of learning and memory impairment was induced by continuous intraperitoneal injection of scopolamine (0.8 mg · kg-1 · d-1), and pranlukast (0.3, 0.6, 1.2 mg · kg-1 · d-1) After 14 days, Morris water maze test and Y-maze test were used to evaluate learning and memory function, and brain acetylcholine (Ach) and acetylcholinesterase (Ach E) levels were measured. Results Pranlukast (0.3 ~ 1.2 mg · kg-1 · d-1) significantly shortened the incubation period of mice in the hidden platform experiment and increased the residence time in the quadrant of the original platform in the space exploration experiment, The number of positions, increase the number of correct response in the Y maze test. Pranlukast also significantly increased Ach content in hippocampus and cortex in mouse brain and decreased AchE activity. Conclusion Pranlukast reduced the learning and memory impairment induced by scopolamine in mice by decreasing AchE activity, increasing Ach content in brain.