论文部分内容阅读
目的:探讨miR-26b在乳腺癌细胞中的表达及其对乳腺癌细胞生物学行为的的影响。方法:比较正常乳腺细胞系MCF-10A及乳腺癌细胞系MCF-7中miR-26b的表达差异。以无处理的MCF-7细胞为空白对照,分别检测MCF-7细胞转染miR-26b模拟物(miR-26b组)、空质粒(阴性对照组)后的miR-26b表达与增殖、迁移、侵袭能力,以及Foxf2的mRNA与蛋白表达的变化。用双荧光素酶报告系统检测miR-26b对MCF-7细胞中Foxf2转录活性的影响。结果:miR-26b在MCF-7细胞中的表达水平明显低于MCF-10A细胞(P<0.05)。与空白对照组和阴性对照组比较,miR-26b组miR-26b mRNA表达水平明显升高、细胞增殖迁移、侵袭能力明显降低,Foxf2的mRNA和蛋白表达量均明显下调(P<0.05)。转染miR-26b模拟物后,MCF-7细胞中Foxf2-3’UTR的转录活性明显抑制(P<0.05)。结论:miR-26b在乳腺癌细胞中表达降低、增加其表达能抑制乳腺癌细胞的恶性生物学行为,机制可能与其下调Foxf2的表达有关。
Objective: To investigate the expression of miR-26b in breast cancer cells and its effect on the biological behavior of breast cancer cells. Methods: The expression differences of miR-26b in normal breast cell line MCF-10A and breast cancer cell line MCF-7 were compared. The untreated MCF-7 cells were used as a blank control to detect the expression of miR-26b in MCF-7 cells transfected with miR-26b mimics (miR-26b group) and empty plasmid (negative control group) Invasion ability, and Foxf2 mRNA and protein expression changes. The effect of miR-26b on Foxf2 transcription activity in MCF-7 cells was examined by dual luciferase reporter system. Results: The expression of miR-26b in MCF-7 cells was significantly lower than that in MCF-10A cells (P <0.05). Compared with the blank control group and the negative control group, miR-26b group miR-26b mRNA expression was significantly increased, cell proliferation and migration, invasion ability was significantly reduced, Foxf2 mRNA and protein expression were significantly decreased (P <0.05). After transfection of miR-26b mimics, the transcriptional activity of Foxf2-3’UTR in MCF-7 cells was significantly inhibited (P <0.05). Conclusion: The expression of miR-26b is down-regulated in breast cancer cells. Increasing the expression of miR-26b may inhibit the malignant biological behavior of breast cancer cells, which may be related to the down-regulation of Foxf2 expression.