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目的探讨芪仙安肠方对三硝基苯磺酸(TNBS)诱导溃疡性结肠炎模型大鼠的疗效及作用机制。方法采用TNBS诱导制备溃疡性结肠炎大鼠模型40只,随机分为模型组、阳性对照组及芪仙安肠方低、中、高剂量组,各8只,并选未造模大鼠8只作为正常对照组。各组均灌胃给药,模型组和正常对照组予0.9%氯化钠注射液,阳性对照组予柳氮磺吡啶(SASP)0.50g/kg,芪仙安肠方低、中、高剂量组分别给予相应剂量(5.0、10.0、20.0g/kg)的芪仙安肠方原液,均每日1次。给药时间从造模后第3日开始,连续14日。造模后第18日观察各组大鼠结肠黏膜损伤指数(CMDI)及黏膜病理组织学情况,并检测免疫球蛋白(Ig)A、IgG、补体3(C3)、补体4(C4)、肿瘤坏死因子-α(TNF-α)、白细胞介素-2(IL-2)及白细胞介素-4(IL-4)含量变化。结果芪仙安肠方低、中、高剂量组结肠黏膜病理损伤明显改善,CMDI均降低,C3、C4及IL-4含量明显提高,IgA、IgG、TNF-α、IL-2含量降低,与模型组比较差异均有统计学意义(P<0.01);芪仙安肠方中、高剂量组治疗后CMDI、C3、C4、IL-2、IL-4、TNF-α含量与正常对照组比较差异均无统计学意义(P>0.05),与阳性对照组比较差异有统计学意义(P<0.01)。结论芪仙安肠方对TNBS诱导的大鼠溃疡性结肠炎疗效确切,中、高剂量尤其显著,其作用机制可能与免疫调节和减轻细胞因子损伤有关。
Objective To investigate the efficacy and mechanism of Qixian Anchang recipe on trinitrobenzene sulfonic acid (TNBS)-induced ulcerative colitis model rats. Methods Thirty forty rats with ulcerative colitis induced by TNBS were randomly divided into model group, positive control group, low-, medium-, and high-dose groups of Qixian Anchang, 8 rats each, and unmodeled rats were selected. 8 Only as a normal control group. Each group was intragastrically administered. The model group and normal control group were given 0.9% sodium chloride injection, and the positive control group was given sulfasalazine (SASP) 0.50 g/kg. The low, medium, and high doses of Qixian Anchang were administered. The corresponding doses (5.0, 10.0, 20.0 g/kg) of the Guixian Anchang recipe were given once daily. The administration time started on the 3rd day after the model was established and continued for 14 days. On the 18th day after modeling, the colonic mucosal injury index (CMDI) and pathological histology of the mucosa were observed in each group, and immunoglobulin (Ig) A, IgG, complement 3 (C3), complement 4 (C4), and tumor were detected. Changes in necrosis factor-α (TNF-α), interleukin-2 (IL-2), and interleukin-4 (IL-4) levels. Results The pathological damage of colon mucosa in the low, middle and high doses of Qixian Anchangfang was significantly improved, the CMDI was decreased, the contents of C3, C4 and IL-4 were significantly increased, and the contents of IgA, IgG, TNF-α and IL-2 were decreased. The difference between the model group was statistically significant (P<0.01); the content of CMDI, C3, C4, IL-2, IL-4, and TNF-α in the middle and high dose group of Qixian Anchangfang was compared with the normal control group. The difference was not statistically significant (P>0.05), and there was a statistically significant difference compared with the positive control group (P<0.01). Conclusion Qixian Anchang recipe is effective for TNBS-induced ulcerative colitis in rats, especially in the medium and high doses. Its mechanism may be related to immune regulation and reduce the damage of cytokines.