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本研究应用流式细胞术(FCM)建立组织因子微颗粒的检测方法,并探讨其在出凝血异常中的临床意义。应用特异性荧光抗体CD142-PE标记组织因子,采用FCM建立组织因子微颗粒的检测方法。测定20例血栓性疾病患者及25例初治急性早幼粒细胞白血病(APL)患者治疗前后的组织因子微颗粒,观察组织因子微颗粒在各组患者中的变化。结果表明,20例血栓性疾病患者组织因子微颗粒〔(123.28±197.03)/μl〕明显高于20例健康对照组〔(33.27±16.14)/μl,P<0.05〕;25例APL患者治疗前组织因子微颗粒为(75.24±104.58)/μl,亦高于正常对照组(P<0.05),经过诱导治疗缓解后组织因子微颗粒水平明显下降至(34.24±25.20)/μl(P<0.01)。在这25例APL患者中,18例合并弥散性血管内凝血(DIC)患者的组织因子微颗粒在治疗前后有明显差异(P<0.05),7例未合并DIC患者治疗前后的组织因子微颗粒水平未见明显变化。结论:采用流式细胞术检测组织因子微颗粒可对血栓性疾病的诊断提供帮助,并对APL的DIC状况与疾病预后做出评估。
In this study, flow cytometry (FCM) to establish tissue factor micro-particle detection method and explore its clinical significance in the coagulation abnormalities. The specific fluorescent antibody CD142-PE was used to label tissue factor, and the detection method of tissue factor micro-particles was established by FCM. Tissue factor particles of 20 patients with thrombotic disease and 25 patients with newly diagnosed acute promyelocytic leukemia (APL) before and after treatment were measured to observe the changes of tissue factor particles in each group. The results showed that 20 cases of thrombotic disease patients with tissue factor particles 〔(123.28 ± 197.03) / μl〕 was significantly higher than the 20 healthy subjects 〔(33.27 ± 16.14〕 / μl, P <0.05〕; 25 cases of APL patients before treatment Tissue factor microparticles were (75.24 ± 104.58) / μl, also higher than that of the normal control group (P <0.05). The level of tissue factor microparticles decreased significantly to (34.24 ± 25.20) / μl after induction therapy (P <0.01) . Among the 25 patients with APL, tissue factor microscopic particles of 18 patients with diffuse intravascular coagulation (DIC) had significant difference before and after treatment (P <0.05), and tissue factor particles before and after treatment in 7 patients without DIC No significant change in level. CONCLUSIONS: The detection of tissue factor micro-particles by flow cytometry may be helpful in the diagnosis of thrombotic diseases. The status of DIC in APL and the prognosis of the disease are also evaluated.