论文部分内容阅读
目的探讨半导体激光和高压静电治疗对糖尿病大鼠血浆内皮素和血管损害的影响。方法选择健康雄性Wistar大鼠50只,取8只作为正常对照组(正常组),其余大鼠腹腔注射链脲佐菌素(STZ)建立糖尿病动物模型后,随机分为糖尿病对照组(模型组)、半导体激光治疗组(激光组)、高压静电治疗组(高压静电组)、高压静电和半导体激光联合治疗组(联合组)。各治疗组给予相应的治疗,每日1次,10次为1个疗程,治疗2个疗程后取各组大鼠静脉血以及心、肾组织标本,检测其血糖、胰岛素、内皮素(ET)并观察组织病理改变。结果模型组大鼠小血管壁增厚,血糖和ET水平增高,胰岛素活性下降,与正常组比较差异有统计学意义(P<0.01)。激光组、高压静电组和联合组治疗后,ET水平明显下降,与模型组比较差异有统计学意义(P<0.01);联合组的胰岛素水平明显高于模型组,差异有统计学意义(P<0.05)。结论以STZ诱导的糖尿病大鼠出现血糖、胰岛素和ET严重失调,心、肾组织小动脉壁增厚、肾小球水肿以及系膜细胞增生。半导体激光和高压静电治疗对糖代谢紊乱所致的血浆ET水平增高和微小血管损害具有预防治疗作用。
Objective To investigate the effects of laser diode and high voltage electrostatic therapy on plasma endothelin and vascular damage in diabetic rats. Methods Fifty healthy male Wistar rats were randomly divided into normal control group (n = 8) and normal control group (n = 8). The other rats were intraperitoneally injected with streptozotocin (STZ) to establish diabetic animal models and then randomly divided into diabetic control group ), Semiconductor laser treatment group (laser group), high voltage electrostatic therapy group (high voltage electrostatic group), high voltage electrostatic and semiconductor laser combination therapy group (combination group). The treatment groups were given the appropriate treatment, once daily, 10 times as a course of treatment, after 2 courses of treatment, venous blood and heart and kidney tissue samples of rats in each group were taken and their blood glucose, insulin, endothelin (ET) And observe the histopathological changes. Results In the model group, the small blood vessel wall was thickened, blood glucose and ET levels were increased, and insulin activity was decreased. There was significant difference between the model group and the normal group (P <0.01). Compared with the model group, the levels of ET in the laser group, the high-voltage electrostatic group and the combined group were significantly decreased (P <0.01); the insulin level in the combined group was significantly higher than that in the model group (P <0.05). Conclusion STZ-induced diabetic rats have serious disorders of blood glucose, insulin and ET, thickening of arterioles in the heart and kidney, glomerular edema and mesangial cell proliferation. Semiconductor laser and high-voltage electrostatic treatment of glucose metabolism disorders caused by increased plasma ET levels and microvascular damage prevention and treatment.