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以水稻幼苗为材料,研究了不同质量浓度(1、100、1 000、3 000μg·L-1)微囊藻毒素(MCs)在胁迫期和恢复期对水稻幼苗根系生长、吸收活力、抗氧化系统的影响以及MCs在水稻根部的积累.结果表明,胁迫处理7 d,MCs在水稻根部的积累量与MCs质量浓度呈正相关.1μg·L-1MCs处理促进了根系生长,根系活力上升,过氧化氢酶(CAT)活性上升有效维持H2O2于正常水平;100μg·L-1MCs处理下,根系生长受抑,根系活力下降,CAT无显著变化;在高质量浓度(1 000μg·L-1、3 000μg·L-1)MCs处理下,不仅根系生长受抑、根系活力下降,且CAT活性受抑,H2O2大量积累,膜质过氧化加剧.相比胁迫期,恢复7 d后各处理组水稻根系MCs的积累量均低于胁迫期.100μg·L-1MCs处理组根系各生长指标、根系活力、丙二醛(MDA)、H2O2和CAT的变幅减小,优于胁迫期,显示出一定程度的恢复,而1 000μg·L-1和3 000μg·L-1MCs处理组,根系生长与根系活力均低于胁迫期,氧化胁迫进一步加剧,表明高质量浓度(1 000μg·L-1和3 000μg·L-1)MCs对水稻根系造成的伤害不可逆.
Rice seedlings were used to study the effects of different concentrations (1, 100, 1 000, 3 000 μg · L -1) of microcystin (MCs) on the root growth, absorption and antioxidant activity of rice seedlings under stress and convalescence. And the accumulation of MCs in the roots of rice.The results showed that the accumulation of MCs in rice roots was positively correlated with the mass concentration of MCs on the 7th day after stress treatment.The treatment with 1μg · L-1MCs promoted root growth, root activity, Under the condition of 100μg · L-1MCs, the growth of root system was inhibited, the root activity decreased, and the CAT did not change significantly. At high concentration (1000μg · L -1, 300μg · L-1) MCs, not only root growth inhibition, root activity decreased, but CAT activity was inhibited, H2O2 accumulation and hyperoxidation increased.Compared with the stress control, (P <0.05) .All the growth indexes, root activity, MDA content, H2O2 and CAT amplitude of 100μg · L-1MCs treatment group were decreased, which were better than the stress period, which showed a certain degree of accumulation Recovery, and 1 000μg · L-1 and 3 000μg · L-1MCs treatment group, root growth and root activity They were lower than the stress period, further exacerbated oxidative stress, indicating that the high concentration (1 000μg · L-1 and 3 000μg · L-1) harm MCs of rice root cause irreversible.