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目的 观察肾病综合征(NS)在强的松治疗前后激素敏感者外周血单个核细胞(PBMC)的细胞凋亡变化,以探讨PBMC凋亡在激素治疗中的意义及与尿蛋白的关系。方法37例激素敏感型NS儿分别于强的松治疗前和治疗后第2、4、6、8周采血,进行细胞凋亡形态学观察,计算百分率。结果 治疗前后2、4、6周比较差异均有显著性(P均<0.01);治疗后外周血PBMC与尿蛋白变化呈正相关关系。结论 激素敏感型NS儿使用足量强的松治疗可诱导外周血PBMC细胞凋亡增加,也诱导其病情缓解,当尿蛋白转阴后诱导细胞凋亡随之减少。
Objective To observe the changes of apoptosis in peripheral blood mononuclear cells (PBMCs) of nephrotic syndrome (NS) before and after prednisone treatment to explore the significance of PBMC apoptosis in hormone therapy and its relationship with urinary protein. Methods Thirty-seven hormone-sensitive NS children were harvested before prednisone treatment and at 2, 4, 6 and 8 weeks after treatment, respectively. Morphological changes of apoptosis were observed and the percentages were calculated. Results There were significant differences between before and after 2, 4 and 6 weeks of treatment (P all <0.01). There was a positive correlation between PBMC and urinary protein after treatment. Conclusion The use of prednisone in hormone sensitive NS children can induce the apoptosis of PBMCs in peripheral blood to increase, and also lead to the remission of the disease. When the proteinuria turns negative, the induction of apoptosis decreases.