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酸性磷酸酶(acid phosphatase)在人体中的分布非常广泛,具有多种不同的分子形式。主要有前列腺酸性磷酸酶(PAP)、组织酸性磷酸酶及红细胞酸性磷酸酶(Erythrocyte acid phosphatase,EAP)三种,其中以PAP活性最高;而EAP则是一组单位点复等位基因控制的,具有遗传多态性的同工酶。1963年Hopkinson首次发现EAP具有多态性。EAP常见的表型有三种:B-B,B-A,A-A。1980年Randall首次应用等电聚焦电泳检测了室温下存放的血痕EAP表型。随后,Divall及Stella分别利用此法检测了血痕EAP表型。1986年日本学者南方首次利用等电聚焦检测了日本人群新鲜血EAP表型;1987年Saha用此法检测了新加坡人群新鲜血EAP表型,两者均未检测血痕EAP表型。本文采用薄层聚丙烯酰胺凝胶等电聚焦
Acid phosphatase is widely distributed in the human body and has many different molecular forms. There are mainly three kinds of prostatic acid phosphatase (PAP), tissue acid phosphatase and erythrocyte acid phosphatase (EAP), of which PAP activity is the highest; while EAP is a group of single point allele-controlled, Isoenzyme with genetic polymorphism. Hopkinson first discovered EAP polymorphism in 1963. There are three common EAP phenotypes: B-B, B-A, A-A. 1980 Randall for the first time using isoelectric focusing electrophoresis stored at room temperature stored EAP phenotype. Subsequently, Divall and Stella were used to detect the bloodstain EAP phenotype. In 1986, Japanese scholars first used the isoelectric focusing to detect the EAP phenotype of fresh blood in the Japanese population. In 1987, Saha used this method to detect the EAP phenotype of fresh blood in the Singaporean population, neither of which tested the EAP phenotype. In this paper, TLC polyacrylamide gel isoelectric focusing