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本实验利用兔大脑中动脉闭塞模型，通过不连续密度梯度超速离心分离出脑微血管（ＭＣＶ）和突触膜（ＳＰＭ），生化法测其Ｎａ＋、Ｋ＋－ＡＴＰ酶活性。结果发现：ＭＣＶＮａ＋、Ｋ＋－ＡＴＰ酶活性在缺血２小时及４小时组高于对照组，与脑组织Ｈ２Ｏ含量之间至正相关，而缺血２４小时组则低于对照组；脑缺血组ＳＰＭＮａ＋、Ｋ＋－ＡＴＰ酶活性明显低于对照组，且与脑组织Ｈ２Ｏ含量之间呈负相关。结果提示：早期脑缺血脑水肿的发生与ＭＣＶ，ＳＰＭＮａ＋、Ｋ＋－ＡＴＰ酶活性变化有关。
In this experiment, MCAO and SPM were isolated by discontinuous density gradient ultracentrifugation using rabbit middle cerebral artery occlusion model. The activity of Na +, K + -ATPase was measured by biochemical method. The results showed that the activities of MCVNa + and K + -ATP were higher in the ischemic group at 2h and 4h than those in the control group, and positively correlated with the content of H 2 O in brain tissue, while those in the 24-h group were lower than those in the control group Group SPMNa +, K + -ATP enzyme activity was significantly lower than the control group, and with a negative correlation between brain tissue H2O content. The results suggest that the occurrence of cerebral ischemia and cerebral edema in early stage is related to the changes of MCV, SPMNa +, K + -ATPase activity.