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目的分析乙型肝炎孕妇HBV血清标志物(HBV-M)、HBV前S1抗原(preS1-Ag)及HBV DNA的相关性,探讨联合检测的临床价值。方法采用化学发光免疫分析法对375例乙型肝炎孕妇血清进行HBV-M的检测,用ELISA法检测preS1-Ag,同时采用荧光定量PCR法检测HBV DNA。结果在不同模式乙型肝炎孕妇血清中,preS1-Ag、HBeAg的检出率分别是61.87%、34.67%;在HBeAg阳性血清中,preS1-Ag检出率为90%,HBeAg阴性血清的检出率为46.94%,两者之间有统计学意义;HBV DNA与HBeAg一致性比较较差,而与preS1-Ag一致性比较较好。结论 HBVpreS1-Ag较HBeAg更敏感地反映了HBV的复制情况,HBV preS1-Ag和HBV DNA有较高的符合率,可作为HBV复制的指标,三者联合检测,可及时阻断乙肝母婴传播,降低婴儿乙肝感染率。
Objective To analyze the correlation between serum HBV markers (HBV-M), preS1-Ag and HBV DNA in pregnant women with hepatitis B and to explore the clinical value of combined detection. Methods Chemiluminescent immunoassay was used to detect HBV-M in serum of 375 hepatitis B patients. PreS1-Ag was detected by ELISA and HBV DNA was detected by real-time PCR. Results The positive rates of preS1-Ag and HBeAg were 61.87% and 34.67% respectively in sera of hepatitis B patients with different patterns of sera. The detection rate of preS1-Ag was 90% in HBeAg-positive sera and that of HBeAg-negative sera Rate was 46.94%, there was statistical significance between the two; HBV DNA and HBeAg consistency is relatively poor, but with preS1-Ag consistency is better. Conclusion HBVpreS1-Ag reflects HBV replication more sensitively than HBeAg. HBV preS1-Ag and HBV DNA have a high coincidence rate, which can be used as an indicator of HBV replication. Combined detection of HBV preS1-Ag and HBV HBV can block the transmission of hepatitis B virus , Reduce the infant hepatitis B infection rate.