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合成了基于分子内荧光能量转移的蒽(An)-四苯基卟啉(TPP)双发色团碘荧光探针1.由于An的荧光光谱与TPP的S吸收带具有较好的重叠,供体An与受体TPP之间可以发生有效的分子内荧光能量转移,以An的最大吸收波长作为激发波长时,由于分子内荧光能量转移,受体TPP发出荧光.当碘与探针分子中的识别基团An作用时,导致探针分子的荧光转导基团TPP荧光淬灭.与An、TPP和An+TPP混合物作敏感材料相比,将探针1固定在PVC膜中制备的敏感膜对碘选择性高、灵敏度好.另外,敏感膜具有很好的重现性、可逆性和稳定性,响应时间小于60s.除Cr2O72-和MnO4-外,食品中常见的无机离子和可能存在的干扰物质不影响碘的测定.在最优条件下,传感器的线性范围为2.04×10-6～2.36×10-2mol/L,检出限为3.30×10-8mol/L.本方法应用于加碘食盐中碘含量的测定,结果满意.
An An-tetrakisoporphyrin (TPP) double chromophore iodofluorescent probe based on intramolecular fluorescence energy transfer was synthesized.1 Because the fluorescence spectrum of An has a good overlap with the S absorption band of TPP, The effective intramolecular fluorescence energy transfer can occur between body An and receptor TPP, and when the maximum absorption wavelength of An is used as the excitation wavelength, the receptor TPP emits fluorescence due to the intramolecular fluorescence energy transfer. When iodine and probe molecules Compared with An, TPP and An + TPP mixtures, the probe 1 was immobilized on the sensitive membrane prepared in PVC membrane by the fluorescence quenching of fluorescent probe TPP. High selectivity to iodine, good sensitivity.In addition, the sensitive membrane has good reproducibility, reversibility and stability, the response time is less than 60s.In addition to Cr2O72- and MnO4-, the common inorganic ions in food and the possible existence Under the optimal conditions, the linear range of the sensor was 2.04 × 10-6 ~ 2.36 × 10-2mol / L with the detection limit of 3.30 × 10-8mol / L. The method was applied to the determination of iodine Determination of iodine in iodized salt with satisfactory results.