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目的分析HBV携带产妇血清与乳汁HBV-DNA变量相关性,为安全哺乳提供科学依据。方法 ELISA法检测HBV携带产妇血清HBV-M,荧光定量PCR检测血清与乳汁HBV-DNA,统计学方法分析两变量相关性。结果以乳汁HBV-DNA小于500拷贝/ml为阴性,其中A组(HBsAg、HBeAg、HBcAb阳性)乳汁阴性率为11.7%(15/128),B组(HBsAg、HBcAb阳性或HBsAg、HBeAg阳性)乳汁阴性率72.3%(11/15),C组(HBsAg、HBAbe、HBcAb阳性)乳汁阴性率94.9%(185/195),三组阴性率比较差异有统计学意义(P<0.05)。248例HBV携带产妇同时检测血清和乳汁HBV-DNA,各组分组情况同上,A组血清HBV-DNA阳性率95.4%(83/87),乳汁HBV-DNA阳性率80.7%(71/87),两者阳性率比较,差异有统计学意义(P<0.05);B组血清HBV-DNA阳性率为46.2%(6/13),乳汁HBV-DNA阳性率30.8%(4/13),差异无统计学意义(P>0.05);C组血清与乳汁HBV-DNA阳性率分别为31.8%(37/148)和4.1%(6/148),差异有统计学意义(P<0.05),每组两变量统计学分析正相关且相关程度高。结论不同感染模式的HBV携带产妇,血清与乳汁HBV-DNA病毒载量不同,HBV携带产妇哺乳前应该检测血清和母乳中的HBV-DNA,以指导母乳安全喂养。
Objective To analyze the correlation between serum HBV-DNA in breast-feeding mothers and HBV-DNA in breast milk and to provide a scientific basis for safe lactation. Methods Serum HBV-M was detected by ELISA in serum and milk of HBV-DNA in pregnant women. Fluorescent quantitative PCR was used to detect HBV-DNA in serum and milk. Statistical analysis was used to analyze the correlation between the two variables. Results The negative rate of breast milk HBV-DNA was less than 500 copies / ml, of which 11.7% (15/128) was negative in group A (HBsAg, HBeAg and HBcAb positive), B (HBsAg, HBcAb positive or HBsAg, HBeAg positive) The negative rate of milk was 72.3% (11/15). The negative rate of milk in Group C (HBsAg, HBAbe, HBcAb positive) was 94.9% (185/195). There was significant difference between the three groups (P <0.05). HBV-DNA of serum and milk was detected in 248 HBV-infected mothers at the same time. The positive rates of serum HBV-DNA in group A were 95.4% (83/87), and the positive rates of HBV-DNA in milk were 80.7% (71/87) in group A, The positive rate of HBV-DNA in group B was 46.2% (6/13), the positive rate of HBV-DNA in milk was 30.8% (4/13), the difference was statistically significant (P <0.05) (P> 0.05). The positive rates of HBV-DNA in serum and milk in group C were 31.8% (37/148) and 4.1% (6/148) respectively, with statistical significance (P <0.05) Two-variable statistical analysis was positively correlated and correlated to a high degree. Conclusions The HBV-DNA viral load in HBV-infected mothers with different infection patterns is different from that in serum and milk. HBV-DNA in serum and breast milk should be tested before breast-feeding in HBV to guide the safe breastfeeding.