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目的分析泰安市2007-2008年度流行性感冒的病原学检测结果,了解泰安市流感病毒亚型分布。方法采集流感样病例(ILI)的咽拭子样品,用狗肾细胞(MDCK)进行病毒分离,采用血凝抑制试验(HI)和RT-PCR进行流感病毒型别鉴定。结果检测国家级流感监测医院ILI咽拭子样品281份,分离出流感病毒94株,阳性分离率为33.45%,经分型鉴定H3N2亚型51株,H1N1亚型1株,B型Yamagata系39株,Victoria系3株。结论2007-2008年度泰安市流感流行的优势毒株为H3N2亚型和B型Yamagata系,同时有H1N1亚型和B型Victoria系毒株的存在。
Objective To analyze the etiological detection results of influenza in Tai’an from 2007 to 2008 and understand the distribution of influenza virus subtypes in Tai’an. Methods Samples of throat swab from influenza-like illness (ILI) were collected and virus isolated from dog kidney cells (MDCK). Influenza type was identified by HI and RT-PCR. Results A total of 281 ILI throat swab samples from the National Influenza Surveillance Hospital were detected and 94 strains of influenza virus were isolated. The positive isolation rate was 33.45%. 51 H3N2 subtypes, 1 H1N1 subtype, 1 Yamagata B 39 strain Strain, Victoria 3 strains. Conclusion The predominant strains of influenza in Tai’an City in 2007-2008 are H3N2 and B type Yamagata strains, with the presence of H1N1 subtype and B type Victoria strain.