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关于产生抗体与杂交瘤细胞生长情况的关系,和融合后产生抗体杂交瘤细胞的均一性及稳定性等方面报导极少.某些作者主张不产生抗体的克隆细胞生长速度超过产生抗体的克隆细胞,但迄今未经实验证明.为了回答这些问题,作者做了两部分试验.一、以不同浓度细胞作融合,然后培养9天,检查产生抗体杂交瘤细胞的形成情况.二、用三种浓度融合后,第11天开始检测,第十四天作有限稀释培养,两周后筛选各孔,用细胞毒试验测定生长孔的上清液.计算每一浓度克隆平均数/孔,并与泊松(Poisson)分
There are few reports on the relationship between antibody production and hybridoma cell growth and on the homogeneity and stability of antibody-producing hybridoma cells.Some authors contend that non-antibody-producing clonal cells grow more rapidly than antibody-producing clonal cells , But so far without experimental evidence.In order to answer these questions, the author did two parts of the experiment.First, to different concentrations of cells for fusion, and then cultured for 9 days to check the formation of antibody hybridoma cells.Second, with three concentrations After fusion, the assay was started on day 11 and limited dilution was performed on the fourteenth day, and after two weeks the wells were screened and the growth supernatants of the wells were assayed by cytotoxicity. The average number of colonies / well was calculated for each concentration, Poisson points