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目的 该研究采用将牙周膜细胞(periodontal ligament cells, PDLCs)培养中经典的组织块法和酶消化法结合起来的联合法进行PDLCs分离培养,旨在寻找一种简单易行的PDLCs培养模式,缩短牙周膜原代细胞培养周期.方法 取25 颗因正畸需要拔除的完整双尖牙,运用联合法进行牙周膜原代细胞的提取培养,计算成功率.结果 采用联合法分离培养可获得PDLCs,所得的PDLCs呈长梭形,符合PDLCs的形态和生物学特征,长势良好,其成功率为40%.结论 运用联合法提取培养PDLCs的方法可行,所得细胞形态及长势良好.“,”Objective To explore a simple and practicable cultivating mode of periodontal ligament cells (PDLCs) to shorten the cultivating period of primary periodontal ligament cells. This study combined tissue cultivation of PDLCs with enzyme digestion and used this combination method to isolate and culture PDLCs. Methods Take 25 premolars which need to extract because of orthodontics, and then use combination method to extract and cultivate the primary periodontal ligament cells. The success rate was calculated. Results Combined method can isolate and cultivate primary periodontal ligament cells. The PDLCs is elongated, and conform to the form and biological characteristics of PDLCs. The PDLCs grow well and the success rate is 40%. Conclusion The way of using combination method to extract and cultivate PDLCs is practicable, and the form and growth of the PDLCs are well.