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选择健康Ｗｉｓｔａｒ大鼠４０只，随机分为４组，每组１０只。静式吸入染毒，剂量分别为０、０．５、２．０和５．０ｍｇ／Ｌ丁二烯，每天４小时，每周６天，共１６周。断头处死大鼠，将头迅速投入液氮内速冻，并取出脑组织，Ｍａｔｓｕｍｕｒａ法制各膜蛋白，Ｂｒａｄｆｏｒｄ法进行蛋白定量，孔雀石绿法测定无机磷（Ｐｉ）．根据Ｐｉ和蛋白质的量，计算出ＡＴＰａｓｅ活性（ｎｍｏｌＰｉ／ｍｇ蛋白１０分钟）。结果表明脑组织神经细胞膜Ｎａ＋／Ｋ＋－ＡＴＰａｓｅ和Ｃａ２＋／Ｍｇ２＋－ＡＴＰａｓｅ活性增高，Ｎｅｕｒｏｆｉｌａｍｅｎｔｍｙｏｓｉｎ－ｔｙｐｅＡＴＰａｓｅ活性降低。提示脑细胞代谢活跃，神经轴突轴浆转运障碍，突触传递受阻。
Forty healthy Wistar rats were randomly divided into 4 groups with 10 rats in each group. Static inhalation was given in doses of 0, 0.5, 2.0 and 5.0 mg / L butadiene, 4 hours a day, 6 days a week for a total of 16 weeks. The rats were killed by decapitation, the head was promptly put into liquid nitrogen and snap frozen. The brain tissues were removed. Matsumura membrane protein was assayed by the method of Bradford. The inorganic phosphorus was determined by the malachite green assay. ATPase activity (nmolPi / mg protein for 10 minutes) was calculated based on the amount of Pi and protein. The results showed that the activity of Na + / K + -ATPase and Ca2 + / Mg2 + -ATPase increased and the activity of Neurofilament myosin-type ATPase decreased in the brain tissue. Tip brain cells active metabolism, axonal axonal transport disorder, blocked synaptic transmission.