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为探讨一氧化氮及其合酶在高胆固醇饮食诱导的动脉粥样硬化中的改变及 Ｌ－ 精氨酸的抗动脉粥样硬化作用，在高胆固醇饮食诱导的动脉粥样硬化模型上，检测了血清总胆固醇、血浆精氨酸、非对称性二甲基精氨酸和氧化型低密度脂蛋白浓度，主动脉一氧化氮合酶活性、一氧化氮生成量及内膜斑块面积。结果发现，高胆固醇饮食对血浆 Ｌ－ 精氨酸水平无明显影响，但血清总胆固醇、血浆非对称性二甲基精氨酸和氧化型低密度脂蛋白浓度显著升高，主动脉一氧化氮合酶活性及一氧化氮生成量分别较正常对照组降低４５ ％ 及３０ ％ （ Ｐ＜ ０ ．０１） ，内膜斑块面积达４２ ．６ ％ ±９ ．２ ％ 。口服 Ｌ－ 精氨酸虽无降低血清总胆固醇、血浆非对称性二甲基精氨酸和氧化型低密度脂蛋白浓度作用，但血浆 Ｌ－ 精氨酸浓度约升高一倍，主动脉一氧化氮合酶活性显著升高，一氧化氮生成量恢复正常（ Ｐ＜ ０ ．０１） ，内膜斑块面积减少至１９ ．５ ％ ±７ ．４ ％ （ Ｐ＜ ０ ．０５） 。结果提示，高胆固醇饮食诱导的动脉粥样硬化兔主动脉一氧化氮合酶－ 一氧化氮系统活性显著受损，动脉粥样硬化的发生发展与该系统活性受损密切相关。内源性一氧化氮生成减少是由于主动脉一氧化氮合酶活性降低所致。 Ｌ－ 精氨酸通?
To investigate the changes of nitric oxide and its synthase in atherosclerosis induced by hypercholesterolemia and the anti-atherosclerosis effect of L-arginine, we measured the level of atherosclerosis induced by high cholesterol diet Serum total cholesterol, plasma arginine, asymmetric dimethylarginine and oxidized low-density lipoprotein concentrations, aorta nitric oxide synthase activity, nitric oxide production and plaque area. The results showed that high cholesterol diet had no significant effect on plasma L-arginine levels, but serum total cholesterol, plasma asymmetric dimethyl arginine and oxidized low-density lipoprotein concentrations were significantly increased, aortic nitric oxide Synthase activity and nitric oxide production were reduced by 45% and 30% respectively (P <0.01), and the plaque area was 42. 6% ± 9. 2%. Although oral L-arginine did not reduce serum total cholesterol, plasma asymmetric dimethyl arginine and oxidized low-density lipoprotein concentrations, but the concentration of plasma L-arginine doubled about aortic Nitric oxide synthase activity was significantly increased, the amount of nitric oxide returned to normal (P <0.01), the plaque area reduced to 19. 5% ± 7. 4% (P <0 .05). The results suggest that high cholesterol diet induced atherosclerosis rabbit aorta nitric oxide synthase - nitric oxide system activity was significantly impaired, the occurrence and development of atherosclerosis and the activity of the system is closely related. Decreased endogenous nitric oxide production is due to decreased aortic nitric oxide synthase activity. L-arginine pass?