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盆载试验表明,2,4-D加田菁固氮根瘤菌处理麦功成功地诱发根部结瘤,其植株生育正常。株高和干重明显高于不固氮参照组(2,4-D加灭活田菁固氮根瘤菌处理)。ARA法证实其根系呈现固氮酶活性;Kjeldahl法表明其植株,特别是根部,全氮产量大多于高于参照组;~(15)N同位素稀释法测得其茎叶~(15)N原子百分超低于不固氮参照组,表明,2,4-D加田菁固氮根瘤菌诱发小麦“类瘤”能固定大气氮。连续4年多次试验表明,2,4-D加田菁固氮瘤处理能使小麦百分之百诱发结瘤,但并非诱发瘤都能固氮。用~(15)N同位素稀释法测定表明,其固氮能力较低,波动甚大。每盆净固氮量约为0.05～18.1 mg纯氮(合0.01～3.87mg N/株),占植株全氮量的2.32％～18.07％。~(15)N同位素稀释法还证实了盆载试验条件下 的田菁固氮根瘤菌的自生固氮活性,其固氮产物能为小麦植株吸收。最后还提出了准确计算结瘤小麦固氮百分率的数学方程式。
Pot experiments showed that 2,4-D plus Nitrogen-fixing Rhizobium meliloti successfully induced root nodulation and its plantlets were normal. The plant height and dry weight were significantly higher than those of the non-nitrogen-fixing reference group (2,4-D plus immobilized Azotobacter). The ARA assay showed that the root system exhibited nitrogenase activity. Kjeldahl method showed that the plant, especially the root and total nitrogen production was mostly higher than that of the reference group. The (15) N isotope dilution Sub-ultra-low nitrogen-free reference group, indicating that 2,4-D plus Nitrogen can Rhizobium nitrogen-induced “tumor” can fix atmospheric nitrogen. Several trials for 4 consecutive years showed that 2,4-D plus Pseudostem Nitrogen treatment could induce 100% wheat nodulation, but not to induce neoplasms to fix nitrogen. With ~ (15) N isotope dilution method showed that its nitrogen fixation capacity is low, volatile. The net nitrogen fixation per pot was about 0.05-18.1 mg of pure nitrogen (0.01-3.87 mg N / plant), accounting for 2.32% -18.07% of the total plant nitrogen. ~ (15) N isotope dilution method also confirmed the autotrophic nitrogen fixation activity of Nitrogen-fixing Rhizobium japonica under pot experiment, and its nitrogen-fixing product could be absorbed by wheat plants. Finally, a mathematical equation for accurately calculating the nitrogen fixation percentage of nodulated wheat was also proposed.