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目的研究虾钳草、叶里含珠水提液对·O-2和·OH自由基清除率(%)的影响以及药理作用。方法用水提取中草药虾钳草、叶里含珠(干品),用AP-TEMED系统产生,用Fenton反应产生·OH自由基,分光光度法测定上述药物对·O-2和·OH清除率(%),并进行对比分析。结果虾钳草、叶里含珠水提液(浓度0.1g/ml生药)及维生素C(浓度为1mg/ml),对·O-2清除率分别为:(25.84±1.59)%、(78.19±5.14)%、(100.00±0.00)%,虾钳草与叶里含珠比较(P<0.01),有显著性差异,但均明显低于维生素C;1.0、0.5、0.25(mL)三种不同剂量的虾钳草水溶液对·O-2清除率分别为:25.84%、12.56%、6.53%,相关系数r=0.9993,有直线关系,三种不同剂量的叶里含珠水溶液对清除率分别为:89.66%、48.24%、25.88%,r=0.9996,维生素C作为标准对照,r=0.9967,均有直线关系。虾钳草对·OH自由基清除率为(66.29±1.28)%。结论虾钳草、叶里含珠水提液均有较高的·O-2和羟自由基清除率,可能与它们的药理作用有关。
OBJECTIVE To study the effects of prawn pith and leaf extract containing water on the scavenging rate of free radicals (%) of O-2 and · OH and their pharmacological effects. Methods The Chinese herbal medicine Shrike shrimps were extracted with water and the beads were contained in the leaves. The products were produced by the AP-TEMED system. · OH radicals were generated by the Fenton reaction. The clearance rates of · O-2 and · OH were determined by spectrophotometry %), And comparative analysis. Results The clearance rates of O-2 were (25.84 ± 1.59)% and (78.19), respectively (P <0.05) ± 5.14)% and (100.00 ± 0.00)%, respectively. There were significant differences between the two species (P <0.01) The results showed that the clearance rates of · O-2 were 25.84%, 12.56%, 6.53% and r = 0.9993, respectively. There was a linear relationship between the concentrations of O-2 and the clearance rate of three solutions : 89.66%, 48.24%, 25.88%, r = 0.9996, vitamin C as a standard control, r = 0.9967, have a linear relationship. Shrimp pike grass · OH radical scavenging rate was (66.29 ± 1.28)%. Conclusion The shrimp prawn grass, leaves contain water extract have higher · O-2 and hydroxyl radical scavenging rate, may be related to their pharmacological effects.