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用Ｔ７噬菌体启动子增加抗体Ｆａｂ的产量。ｐ３－３Ｇ９是由抗胃癌鼠单抗３Ｇ９构建的噬菌体抗体表达载体，其Ｆｄ和Ｋ链各由一个Ｌａｃ启动子控制表达，通过ＤＮＡ重组将其改造为单启动子双基因的可溶性Ｆａｂ段表达载体ｐ３Ｇ９－Ｌ，再将Ｆｄ和Ｋ基因转移到质粒ｐＴ７中，置子Ｔ７噬菌体启动子的下游，形成新的Ｆａｂ表达载体ｐＴ７－３Ｇ９。通过免疫印迹实验证明了ｐ３Ｇ９－Ｌ与ｐＴ７－３Ｇ９均有可溶比抗体Ｆａｂ的表达，将两者的表达量进行了比较，后者略高于前者。初步尝试了利用强启动子系统表达可溶性抗体Ｆａｂ，证明ｐＴＴ噬菌体表达载体系统可用于Ｆ３ｂ的分泌型表达，但是表达量的增加并不理想，还有待于进一步探索。
Use of the T7 phage promoter increases the production of antibody Fabs. p3-3G9 is a phage antibody expression vector constructed by the anti-gastric cancer mouse monoclonal antibody 3G9. The Fd and K chains are each controlled by a Lac promoter. The p3-3G9 is transformed into the soluble single-promoter soluble Fab fragment expression vector p3G9-L. The Fd and K genes were then transferred into plasmid pT7 downstream of the T7 phage promoter to form the new Fab expression vector pT7-3G9. The results of Western blotting showed that both p3G9-L and pT7-3G9 expressed soluble Fab, and their expression levels were slightly higher than the former. Preliminary attempts to express the soluble antibody Fab using a strong promoter system proved that the pTT phage expression vector system can be used for the secretion of F3b expression, but the increase in expression is not ideal, remains to be further explored.