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目的观察癫清片对戊四唑(PTZ)点燃大鼠脑组织学损害特点。方法取按照One’s分级标准造模成功的癫大鼠60只,随机分为模型对照组;癫清片高、中、低剂量组;癫宁片组及苯妥英钠片组,连续灌胃给药28 d,处死后脑标本制成4μm层厚的横轴位切片,HE染色,光镜下观察各组海马的CA1、CA2、CA3、CA4的组织学改变,并选择组织学损害最明显的区域在高倍镜下(40×10)计数神经元的丢失。结果模型对照组总的神经细胞数量减少,变性神经细胞可占锥体细胞的83%,各给药组与模型组相比,均可增加总的神经细胞数量,变性神经细胞比率减少,其中癫清片高剂量组与苯妥英钠片组表现相似。结论PTZ可使海马的CA1、CA2、CA3、CA4的大量神经细胞变性坏死;癫清片具有增加锥体层总体神经细胞和正常神经细胞数量的作用。
Objective To observe the characteristics of epilepsy on the histological damage of pentylenetetrazole (PTZ)-induced cerebral ischemia in rats. Methods A total of 60 epileptic rats successfully modeled according to One’s grading standard were randomly divided into model control group; epilepsy clear tablets high, middle and low dose groups; epilepsy and phenytoin tablets group, continuous gavage After 28 days of administration, the brain specimens were made into 4 μm-thick transverse axial slices after being sacrificed. HE staining was performed to observe the histological changes of CA1, CA2, CA3, and CA4 in hippocampus of each group under light microscope, and the most obvious histological damage was selected. The loss of neurons was counted at high magnification (40 x 10) in the area. Results The total number of nerve cells in the model control group decreased, and the denatured nerve cells accounted for 83% of pyramidal cells. Compared with the model group, each administration group can increase the total number of nerve cells, and the ratio of degenerated nerve cells decreased. The high-dose Ganqing Tablets showed similar performance to the phenytoin sodium tablets. Conclusion PTZ can degenerate and necrosis a large number of neurons in CA1, CA2, CA3 and CA4 of hippocampus; epilepsy clear tablets can increase the number of neurons and normal nerve cells in the pyramidal layer.