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目的选用ATCC43300标准菌株,建立MRSA感染的大鼠心内膜炎模型。方法大鼠经右侧颈总动脉插管至左心室,术后24 h,尾静脉注射10~3、10~4和10~5 CFU/只感染量的ATCC43300菌液,分别在感染后的1、3、5和7 d处死大鼠,采集心脏赘生物、脾脏和肾脏进行菌落计数,同时设非手术感染组和手术非感染组作为对照。结果感染1 d后,心脏插管大鼠瓣膜处均有赘生物形成,严重者甚至形成壁性赘生物,且随感染菌浓度的增加,心内膜炎感染程度加剧。10~3 CFU/只感染量组、10~4 CFU/只感染量组、10~5 CFU/只感染量组,赘生物细菌计数的对数值分别为7.04±1.38、9.32±1.22、9.78±1.03;感染3 d后,细菌性心内膜炎感染最为严重,细菌计数的对数值最高可达10.03±0.71;随后的5~7 d,感染程度随感染量均有不同程度的下降,10~3 CFU/只感染量组和10~4 CFU/只感染量组,分别下降至6.87±0.63和8.23±1.05;脾脏和肾脏的感染趋势与心脏大致相同,但感染程度低于心脏。手术非感染组和非手术感染组则无赘生物的形成。结论应用该方法建立的模型具有可操作性强、简单、稳定、重复性好和成功率高的特点,更加适用于抗菌药物机制、新药筛选评价及心内膜炎相关性疾病的研究。
Objective To select ATCC43300 standard strain to establish MRSA-infected rat endocarditis model. Methods The rats were intubated to the left ventricle via the right common carotid artery. After 24 h of operation, 10 to 3, 10 to 4 and 10 to 5 CFU / The rats were sacrificed on days 3, 5 and 7, and the colonies of heart, spleen and kidney were collected for colony counting. At the same time, non-surgical infection group and non-surgical infection group were used as control. Results In 1 d after infection, the neoplasms were formed in the valvular heart of rats with cardiac catheterization. In severe cases, even walled neoplasms were formed. With the increase of the concentration of infectious bacteria, the degree of endocarditis infection was aggravated. The logarithm of bacterial count of neoplasms were 10.04 CFU / infection group, 10-4 CFU / infection group and 10-5 CFU / infection group, respectively. The logarithm of bacterial counts was 7.04 ± 1.38,9.32 ± 1.22,9.78 ± 1.03 After 3 days of infection, bacterial endocarditis infection was the most serious. The logarithm value of bacteria count was up to 10.03 ± 0.71. From the next 5 to 7 days, the infection degree decreased with the degree of infection, while 10 ~ 3 CFU / infected group and 10 ~ 4 CFU / infected group decreased to 6.87 ± 0.63 and 8.23 ± 1.05 respectively. The spleen and kidney showed the same trend of infection as the heart, but the infection level was lower than that of the heart. Neoplasms were not found in the non-surgical and non-surgical groups. Conclusion The model established by this method has the characteristics of strong operability, simple, stable, good repeatability and high success rate. It is more suitable for the study of antimicrobial agents, screening of new drugs and endocarditis-related diseases.