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12 5 I-神经生长因子 (nerve growth factor,NGF)用氯胺 T法制备 ,采用同位素示踪结合三氯醋酸(TCA )沉淀法 ,测定小鼠血浆 NGF的浓度 ,并研究其在体内的分布与排泄。结果表明 ,静注 1 2 5 I- NGF,小鼠体内代谢符合二房室分布模型 ;肌注 1 2 5 I- NGF,小鼠体内代谢符合一房室分布模型。静注 1 2 5 I- NGF2 5 μg.kg- 1 ,测得消除半衰期 (t1 / 2 β)为 4.431h。肌注 1 2 5 I- N GF 75、2 5、10、3.3μg.kg- 1 ,测得消除半衰期 (t1 / 2 β)分别为 2 .135、2 .70 3、3.12 7、4.2 12 h,平均达峰时间 (tmax)为 0 .5 83h。测得 1 2 5 I- N GF平均血浆清除率 (CL)为 0 .2 77L.h- 1 .kg- 1 ,表观分布容积 (Vd)为 1.0 39L.kg- 1 ,体内平均滞留时间 (MRT)为 3.2 38h。1 2 5 I- NGF在组织器官内的分布速率以颈上神经节分布最快 ,大脑、小脑、脊髓分布极低。1 2 5 I- NGF主要经尿液和粪便排泄 ,注射后 72 h内 ,小鼠粪尿中放射性总排泄量为 80 .36 % ,其中尿排泄量占 73.87%
The nerve growth factor (NGF) was prepared by chloramine T method. The plasma concentration of NGF was determined by isotope tracing combined with trichloroacetic acid (TCA) precipitation method and its distribution in vivo With excretion. The results showed that the mice were metabolized in a two-compartment model with 1,25 I-NGF intravenously. Mice were intramuscularly injected with 125 I-NGF and the metabolism in the mice was consistent with a one-compartment distribution model. Intravenous 1 2 5 I-NGF2 5 μg.kg-1, measured elimination half-life (t1 / 2 β) was 4.431h. Intramuscular injection 1 2 5 I-N GF 75,2 5,10,3.3μg.kg-1, measured elimination half-life (t1 / 2 β) were 2.135,2 .70 3,3.12 7,4.2 12 h , The average peak time (tmax) was 0.53 83h. The mean plasma clearance (CL) of 125 I-N GF was measured at 0.277 L · h -1 · kg -1 and the apparent volume of distribution (Vd) was 1.0 39 L · kg -1. The mean in vivo residence time ( MRT) was 3.2 38h. 1 2 5 I-NGF in the distribution of tissues and organs to the distribution of the superior cervical ganglia, cerebrum, cerebellum, spinal cord distribution is extremely low. 1 2 5 I-NGF was mainly excreted in the urine and feces, within 72 h after injection, the total radioactive excretion of mouse excrement was 80.36%, of which urinary excretion accounted for 73.87%