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目的:探讨微小RNA-92a-3p （micro RNA-92a-3p, miR-92a-3p）靶向调控PTEN对神经母细胞瘤（neuroblastoma，NB）细胞增殖、侵袭和迁移的影响。方法:采用miR-92a-3p mimics及其抑制基因（inhibitor）分别转染至SH-SY5Y细胞，根据实验需要分为miR-92a-3p过表达组、NC过表达组、miR-92a-3p抑制组、NC抑制组，采用实时荧光定量聚合酶链反应（real time fluorescent quantitative polymerase chain reaction，qRT-PCR）检测转染后细胞内miR-92a-3p的表达水平；采用CCK-8检测实验、细胞克隆形成实验、细胞划痕实验、细胞侵袭实验检测miR-92a-3p对细胞增殖、侵袭和迁移能力的变化；采用基因软件预测miR-92a-3p的靶基因并用双荧光素酶报告体系进行验证；最后采用qRT-PCR和蛋白质印迹法检测过表达miR-92a-3p和抑制miR-92a-3p后对PTEN/PI3K/AKT信号通路相关分子的表达情况。结果:qRT-PCR结果显示miR-92a-3p过表达组的表达含量（65.73±20.07）比miR-92a-3p抑制组的表达含量（0.33±0.02）显著增高，且差异有统计学意义（n t=5.64，n P=0.005）。CCK-8检测实验显示，miR-92a-3p过表达组能促进SH-SY5Y细胞的增殖活性（n P均<0.001 ），miR- 92a-3p抑制组则能有效抑制SH-SY5Y细胞的增殖活性（n P=0.031，n P=0.012，n P<0.001，n P<0.001）。细胞克隆形成实验结果显示，miR-92a-3p过表达组的细胞克隆数为（210±19）个，高于NC过表达组的细胞克隆数（144±5）个，组间比较差异有统计学意义（n P=0.005）；miR-92a-3p抑制组的细胞克隆数为（83±6）个，低于NC抑制组的细胞克隆数（137±13）个，组间比较差异有统计学意义（n P＝0.003）。细胞划痕实验及细胞侵袭实验结果显示，miR-92a-3p过表达组细胞迁移愈合率为（90.37±0.67）%，高于miR-92a-3p抑制组（41.03±0.56）%，组间比较差异有统计学意义（n P<0.001）；miR-92a-3p过表达组细胞穿膜数量为（106.80±9.28）个，高于miR-92a-3p抑制组（33.40±7.56）个，组间比较差异有统计学意义（n P<0.001）。qRT-PCR和蛋白质印迹法结果显示，与NC过表达组相比，miR-92a-3p过表达组PTEN的mRNA表达量（0.43±0.02）和蛋白水平明显降低，而PI3K mRNA表达量（2.00±0.10）、AKT mRNA表达量（1.41±0.19）和各自蛋白水平明显升高，且差异均有统计学意义（n P<0.001、n P< 0.001和n P=0.028）；miRNA-92a-3p抑制组可逆转上述作用，且差异均有统计学意义（n P＝0.043、n P= 0.046和n P<0.001）。n 结论:PTEN基因是miR-92a-3p的靶基因，miR-92a-3p可能通过促进NB细胞增殖、促进NB细胞侵袭、促进NB细胞迁移和抑制PTEN mRNA表达引发PTEN蛋白水平的降低。“,”Objective:To explore the effects of microRNA- 92a- 3p (miR- 92a- 3p) on proliferation, invasion and migration of neuroblastoma (NB) cells and verify its effect on PTEN in NB cells and its regulatory mechanism.Methods:miR-92a-3p mimics and its inhibitor were transfected into SH-SY5Y cells. According to experimental requirements, sh-SY5Y cells were divided into four groups of miR-92a-3p overexpression, NC overexpression, miR-92a-3p inhibition and NC inhibition. Real-time fluorescent quantitative polymerase chain reaction (qRT-PCR) was employed for detecting the expression level of miR- 92a- 3p in transfected cells. CCK- 8, cell clonogenesis, cell scratch and cell invasion assays were utilized for detecting the changes of miR-92a-3p on cell proliferation, invasion and migration. The target genes of miR- 92a- 3p were predicted by gene software and verified by dual luciferase reporting system. Finally qRT-PCR and Western blot were used for detecting the expression of PTEN/PI3K/AKT signaling pathway related molecules after an overexpression of miR-92a-3p and an inhibition of miR- 92a- 3p.Results:QRT-PCR revealed that the expression level of miR- 92a- 3p overexpressed group (65.73±20.07) was significantly higher than that of miR- 92a- 3p inhibited group(0.33±0.02) and the difference was statistically significant (n t=5.64, n P=0.005). CCK-8 assay showed that miR-92a-3p overexpression group could promote the proliferation activity of SH-SY5Y cells (all n P< 0.001) , while miR-92a-3p inhibition group could effectively inhibit the proliferation activity of SH-SY5Y cells (n P=0.031, n P=0.012, n P<0.001,n P<0.001). The results of cell cloning formation experiment showed that the number of cell clones in miR-92a-3p overexpression group was (210±19) , which was higher than that in NC overexpression group (144±5) , and the difference between groups was statistically significant (n P=0.005). The number of cell clones in miR-92a-3p inhibition group was (83± 6) , which was lower than that in NC inhibition group (137±13) , and the difference was statistically significant (n P=0.003). The assay results of cell scratch and cell invasion showed that the migration healing rate of miR-92a-3p group was (90.37±0.67) % and it was higher than that of inhibition group(41.03±0.56) %. The inter-group difference was statistically significant (n P<0.001). The number of cells penetrating matrix glue in overexpressed miR- 92a- 3p group was (106.80±9.28). It was higher than that in inhibited group (33.40±7.56) and the difference was statistically significant (n P<0.001). qRT-PCR and Western blot results showed that mRNA expression level and protein level of PTEN declined markedly in miR- 92a- 3p group. The expression levels of PI3K mRNA (2.00±0.10) , AKT mRNA (1.41±0.19) and their protein levels spiked significantly with statistical significance (n P< 0.001 ,n P<0.001 , P=0.003). Inhibition of miR- 92a- 3p group reversed the above effects and the differences were statistically significant (n P=0.043, n P=0.046, P<0.001).n Conclusions:PTEN gene is a target gene of miR- 92a- 3p. And miR- 92a- 3p may lower PTEN protein level by promoting NB cell proliferation, NB cell invasion, NB cell migration and inhibiting PTEN mRNA expression.