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比较不同生长年限三七的不同部位中人参皂苷Rg1、Rb1的含量变化规律。以高效液相色谱-蒸发光散射检测(HPLCELSD)法,采用YMC-Pack Pro C18色谱柱(250 mm×4.6 mm,5μm),流动相为水(含0.5%醋酸)-乙腈梯度洗脱,流速为1.5 mL/min;检测不同生长年限三七的不同部位中人参皂苷Rg1、Rb1的含量。不同生长年限三七中人参皂苷Rg1、Rb1的含量存在明显差异。所得数据可为三七的采收及合理利用提供参考。
The changes of content of ginsenosides Rg1 and Rb1 in different parts of Panax notoginseng at different growth stages were compared. The samples were separated on a YMC-Pack Pro C18 column (250 mm × 4.6 mm, 5 μm) using a mobile phase consisting of water (containing 0.5% acetic acid) -acetonitrile gradient by HPLCELSD. The flow rate 1.5 mL / min. The contents of ginsenosides Rg1 and Rb1 in different parts of Panax notoginseng at different growth stages were detected. There were significant differences in the contents of ginsenosides Rg1 and Rb1 in different growth stages of Panax notoginseng. The data obtained for Panax notoginseng harvest and rational use of reference.