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目的 探讨 p2 7kip1、CyclinD1在卵巢癌发生、发展方面的意义。 方法 应用免疫组化染色及半定量分析的方法 ,检测 5 0例卵巢癌、19例卵巢良性上皮肿瘤、13例正常卵巢组织中的p2 7kip1、CyclinD1表达 ,及它们与上皮组织的良恶性、病理组织分级、临床分期的相关性。结果 正常卵巢组和卵巢良性肿瘤组间 ,p2 7、CyclinD1表达无明显差异 ;p2 7在正常卵巢组织和卵巢良性上皮肿瘤中高表达 ,在卵巢癌中表达降低 (P <0 0 5 ) ,且随着肿瘤分级、分期增高 (恶性程度增高 ) ,阳性表达率逐渐下降 ;而CyclinD1的表达则相反 ;两者在肿瘤中的表达呈负相关。结论 p2 7kip1表达下降、CyclinD1过表达可能在卵巢癌的发生发展中起重要作用 ,检测 p2 7kip1、CyclinD1在卵巢癌中的表达可预测该肿瘤生物学行为特征 ,可以作为预后指标
Objective To investigate the significance of p27kip1 and CyclinD1 in the genesis and development of ovarian cancer. Methods The expressions of p27kip1 and CyclinD1 in 50 ovarian cancer tissues, 19 benign epithelial ovarian tumors and 13 normal ovarian tissues were detected by immunohistochemistry and semi-quantitative analysis. Tissue grading, clinical staging of relevance. Results There was no significant difference in the expression of p27 and CyclinD1 between normal ovarian group and benign ovarian tumor group. The positive expression of p27 in normal ovarian tissues and benign ovarian epithelial tumors was lower than that in ovarian cancer (P <0.05) The tumor grade, stage increased (increased degree of malignancy), the positive expression rate decreased gradually; while the expression of CyclinD1 was the opposite; the expression of the two in tumor was negatively correlated. Conclusions The expression of p2 7kip1 is down-regulated and overexpression of CyclinD1 may play an important role in the development of ovarian cancer. The expression of p27kip1 and CyclinD1 in ovarian cancer may predict the biological behavior of the tumor and may be used as a prognostic indicator