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Objective:To study the inhibition of proliferation of breast cancer by small interfering RNA(siRNA) targeting human prolactin(hPRLR) and the underlying mechanisms.Methods:The siRNA targeting hPRLR was chemically synthesized and transfected into MCF-7 cells,the expression of hPRLR was analyzed by real-time quantitive PCR,cell growth inhibition was measured with MTT assay,cell cycle of the transfected cells was examined by flow cytometry,meanwhile,expression of cyclin D1 was tested by semi-quantitative RT-PCR.Results:24 h after transfection with 100 nmol/L siRNA-PRLR,the expression of hPRLR mRNA was suppressed by 65%,cells in G1 phase increased,but cells in S phase decreased.Down regulated hPRLR expression exhibited significant inhibition in cell proliferation.And the expression of cyclin D1 was down regulated.Conclusion:The results indicate that siRNA-hPRLR is a useful tool for silencing hPRLR expression and inhibiting cell proliferation in breast cancer MCF-7 cell line,and it may be a possible new approach for breast cancer gene therapy.
Objective:To study the inhibition of proliferation of breast cancer by small interfering RNA(siRNA) targeting human prolactin(hPRLR) and the underlying mechanisms.Methods:The siRNA targeting hPRLR was chemically synthesized and transfected into MCF-7 cells,the expression of hPRLR Was analyzed by real-time quantitive PCR, cell growth inhibition was measured with MTT assay, cell cycle of the transfected cells was examined by flow cytometry, meanwhile, expression of cyclin D1 was tested by semi-quantitative RT-PCR.Results:24 h After transfection with 100 nmol/L siRNA-PRLR, the expression of hPRLR mRNA was suppressed by 65%, cells in G1 phase increased, but cells in S phase decreased. Down regulated hPRLR expression exhibited significant inhibition in cell proliferation.And the expression of Cyclin D1 was down regulated.Conclusion:The results indicate that siRNA-hPRLR is a useful tool for silencing hPRLR expression and inhibiting cell proliferation in breast cancer MCF-7 cell line,and it may be a Possible new approach for breast cancer gene therapy.