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目的研究抗ＨＥＲ２／ｎｅｕ胞外区的特异性抗体对ＳＫＢＲ３乳腺癌细胞株酪氨酸磷酸化及细胞增殖的影响。方法Ｗｅｓｔｅｒｎ印迹法及抗体介导的特异性细胞增殖试验；筛选乳腺癌病人抗ＨＥＲ２／ｎｅｕ抗体阳性血清ＩｇＧ的ＥＬＩＳＡ方法。结果试验发现单抗ｃ－ｎｅｕ－５对ＳＫＢＲ３细胞ｐ１８５酪氨酸磷酸化有明显促进作用，但对细胞增殖的影响不明显；而单抗ｃ－ｎｅｕ－２对ＳＫＢＲ３细胞ｐ１８５酪氨酸磷酸化及细胞增殖均有明显作用。在此基础上，收集乳腺癌病人血清１４份，分离纯化其血清ＩｇＧ，并经ＥＬＩＳＡ方法筛选出５份乳腺癌病人抗ＨＥＲ２／ｎｅｕ抗体阳性血清ＩｇＧ。细胞增殖试验发现该５份标本中有３份对ＳＫＢＲ３细胞增殖有明显抑制作用。选其中１份抑制增殖的阳性血清ＩｇＧ进一步研究其对ＳＫＢＲ３细胞ｐ１８５酪氨酸磷酸化的影响，结果发现该病人血清ＩｇＧ对细胞酪氨酸磷酸化也有抑制作用。结论抗ＨＥＲ２／ｎｅｕ的特异性抗体可能通过抑制细胞的信号传递系统而抑制肿瘤生长。
Objective To investigate the effect of specific antibodies against HER2/neu extracellular domain on tyrosine phosphorylation and cell proliferation in SKBR3 breast cancer cell lines. Methods Western blotting and antibody-mediated specific cell proliferation assays; screening ELISA for anti-HER2/neu antibody positive serum IgG in breast cancer patients. The results showed that the c-neu-5 monoclone promoted the tyrosine phosphorylation of p185 in SKBR3 cells, but had no obvious effect on cell proliferation, but the p-tyrosine phosphorylation of p185 in SKBR3 cells was inhibited by c-neu-2. And cell proliferation have a significant effect. On this basis, 14 serum samples from breast cancer patients were collected, serum IgG was isolated and purified, and 5 anti-HER2/neu antibody-positive serum IgG were screened by ELISA. Cell proliferation assays revealed that 3 of the 5 specimens significantly inhibited the proliferation of SKBR3 cells. One of the positive serum IgGs inhibiting proliferation was selected to further investigate its effect on tyrosine phosphorylation of p185 in SKBR3 cells, and it was found that serum IgG of this patient also inhibited tyrosine phosphorylation of cells. Conclusion Anti-HER2/neu specific antibodies may inhibit tumor growth by inhibiting the cell’s signaling system.