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为了培育出能够在内蒙古地区大面积推广的优良三叶草品种,以高加索三叶草(2n=6×=48)为母本,白三叶(2n=4×=32)为父本,通过远缘杂交及胚拯救技术得到了杂种F_1代,选择9个杂交F_1代稳定株系,以亲本为对照,采用ISSR分子标记技术分析其遗传差异性。结果表明:16个适宜引物共扩增出111条条带,多态性条带72条,多态性条带百分率为64.86%;11个供试材料间的遗传距离变幅为0.073 4~0.477 1,平均值为0.241 2;9个杂种F1代的株系与父本白三叶之间的遗传距离较大,遗传相似系数较小,表明9个杂种F_1代的株系表达了更多源于母本的遗传信息。
In order to breed fine clover cultivars that can be widely popularized in Inner Mongolia, the white clover (2n = 4 × = 32) was used as the female parent of Caucasian clover (2n = 6 × = 48) Embryo rescue technology was hybrid F_1 generation, selection of nine hybrid F_1 stable strains, the parents as a control, using ISSR molecular markers to analyze the genetic differences. The results showed that 111 bands and 72 polymorphic bands were amplified by 16 suitable primers, the percentage of polymorphic bands was 64.86%. The genetic distance between 11 tested cultivars varied from 0.073 4 to 0.477 1 with an average of 0.241 2. The genetic distance between 9 hybrid F1 lines and male white clover was larger and the genetic similarity coefficient was smaller, which indicated that 9 hybrid F1 lines expressed more sources Genetic information in the mother.