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本文用扫描电镜术(SEM)研究了合成和自然颗粒的吸收和转运过程。方法:将20只大鼠分成两组:1.电子显微镜研究:10只大鼠禁食24h并麻醉,开腹在上段空肠选择四个发育较好的Peyer淋巴集结,封闭此段肠襻,并维持完整的肠系膜血供。分三组动物,分别为:(1)将直径为0.5μm的1%的乳胶粒悬液注于肠腔;(2)注入同样浓度和剂量的乳胶悬液,但颗粒直径为1μm;(3)注入生理盐水以作对照。上述吸收过程完成后,处死大鼠并取其相应的肠襻,组织经固定镀金,然后行电镜扫描。2.用光镜行组织形态检查,研究颗粒的吸收过程。动物分组情况与电镜扫描术相同。组织用偏振光学显微镜进行研究。
In this paper, the absorption and transport of synthetic and natural particles were investigated by scanning electron microscopy (SEM). METHODS: Twenty rats were divided into two groups: 1. Electron microscopy study: Ten rats were fasted for 24 hours and anesthetized. Four well-developed Peyer’s lymphatic patches were selected in the upper jejunum, and the intestine was closed. Maintain a complete mesenteric blood supply. The animals were divided into three groups: (1) a 1% latex suspension with a diameter of 0.5 μm was injected into the intestinal lumen; (2) a latex suspension of the same concentration and dosage was injected but with a particle diameter of 1 μm; ) Into normal saline for comparison. After the above absorption process was completed, the rats were sacrificed and their corresponding bowel loops were taken. The tissues were fixed with gold plating and scanned electron microscopically. 2. Light microscopy examination of tissue morphology, particle absorption process. Animal grouping is the same as for electron microscopy. Tissues were studied using polarized light microscopy.