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用链脲佐菌素 (STZ)作用于离体胰岛β细胞 2 4 h,一氧化氮 (NO)、脂质过氧化产物丙二醛(MDA)生成增多 ,胰岛细胞膜流动性下降 ,微粘度增高 ,同时胰岛素分泌功能降低 ,细胞存活率下降。预先给高浓度褪黑素 (10 μmol/ L)处理 2 4 h者 ,可减少 NO、MDA的释放 ,提高超氧化物歧化酶的水平 ,使胰岛细胞的氧化损伤减轻 ,膜微粘度降低 ,膜流动性增加 ,细胞存活率显著提高。但低浓度褪黑素 (10 nmol/ L)无此作用。提示高浓度褪黑素对 STZ所致的胰岛 β细胞损伤有一定的保护作用。
With streptozotocin (STZ) acting on islet β cells in vitro for 24 h, the production of nitric oxide (NO) and malondialdehyde (MDA), a product of lipid peroxidation, decreased and the fluidity of islet membrane decreased and the microviscosity increased , While insulin secretion decreased, cell survival decreased. Pretreatment with high concentration of melatonin (10 μmol / L) for 24 h reduced the release of NO and MDA, increased the level of superoxide dismutase, reduced the oxidative damage of islet cells and decreased the microviscosity of the membrane, Increased mobility, cell survival significantly increased. However, low concentrations of melatonin (10 nmol / L) did not. These results suggest that high concentrations of melatonin may have a protective effect on pancreatic β-cell injury induced by STZ.