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目的:观察急性低氧和间断低氧习服对大鼠肝、肾组织内红细胞生成素(EPO)及其调控蛋白低氧诱导因子-1α(HIF-1α)基因表达的影响。方法:用低压舱模拟高原低氧环境,进行3km 2周,5km 两周低氧训练,使大鼠达到低氧习服状态后,采用 Northern 斑点杂交研究间断低氧习服前后,8km 4h 低氧处理的大鼠肝、肾组织内 EPO、HIF-1α基因表达的变化。结果:与常氧对照组相比,急性低氧处理的大鼠肝、肾组织内 EPO 基因表达都明显增强(P<0.05～0.01);而间断低氧习服后,同样的低氧处理的大鼠肝、肾组织中 EPO 基因表达都明显低于急性低氧组(P<0.01),mRNA 含量与常氧对照组水平差异不显著(P>0.05)。急性低氧处理的大鼠肝组织中,HIF-1α基因表达水平明显高于常氧对照组和间断低氧习服组(P<0.01),而在不同处理的肾组织中,该基因表达水平差异不明显(P>0.05)。结论:急性低氧能够刺激肝肾组织内 EPO 基因表达,而间断低氧习服的大鼠耐低氧能力明显增强,肝、肾组织内 EPO 基因表达水平恢复到常氧对照组水平。HIF-1α在低氧影响 EPO 基因表达的过程中可能起着重要的调节作用。
Objective: To observe the effects of acute hypoxia and intermittent hypoxic acclimatization on the expression of erythropoietin (EPO) and its regulatory protein hypoxia inducible factor-1α (HIF-1α) in rat liver and kidney. Methods: The hypoxia environment in the plateau was simulated by low pressure cabin. After hypoxia training of 3km 2 weeks and 5km for 2 weeks, the rats were exposed to hypoxia. Northern blotting was used to study the hypoxia of 8km 4h Treatment of rat liver and kidney tissue EPO, HIF-1α gene expression changes. Results: Compared with normoxia control group, the expression of EPO gene in liver and kidney of acute hypoxia rats was significantly increased (P <0.05 ~ 0.01), while the same hypoxia-treated The expression of EPO gene in liver and kidney of rats was significantly lower than that of acute hypoxia group (P <0.01). The mRNA level of EPO gene was not significantly different from that of normoxia control group (P> 0.05). The expression level of HIF-1α in acute hypoxia-treated rat liver tissue was significantly higher than that in normoxia control group and intermittent hypoxic preconditioning group (P <0.01), while in different treatment groups, the gene expression level The difference was not significant (P> 0.05). CONCLUSION: Acute hypoxia can stimulate the expression of EPO gene in liver and kidney tissues. However, the hypoxia tolerance ability of rats in hypoxic preconditioning is obviously enhanced. The expression of EPO gene in liver and kidney is recovered to the level of normoxia control group. HIF-1α may play an important regulatory role in hypoxia-induced EPO gene expression.