论文部分内容阅读
应用核酸杂交技术（ｄｏｔ－ｂｌｏｔ）分析了癌基因。－ｍｙｃ、 Ｎ－ｒａｓ和抑癌基因 ｐ５３在骨髓增生异常综合征（ＭＤＳ）和急非淋白血病（ＡＮＬＬ）中的扩增情况。结果发现，有 ３ 例（３３％）的 ＭＤＳ存在 ｐ５３抑癌基因的 ２倍扩增，本实验中 ｐ５３抑癌基因的扩增发生在 ＭＤＳ的较晚期阶段； １例 ＡＮＬＬ中存在癌基因 ｃ－ｍｙｃ和抑癌基因 ｐ５３的共扩增，且发现扩增与 ＭＤＳ的分型及 ＡＮＬＬ的临床病情有一定的相关性。
Oncogenes were analyzed using dot-blot. -myc, N-ras and tumor suppressor gene p53 in myelodysplastic syndromes (MDS) and acute non-lymphoid leukemia (ANLL). The results showed that 3 cases (33%) of MDS exist two-fold amplification of p53 tumor suppressor gene in this experiment, p53 tumor suppressor gene amplification occurs in the more advanced stages of MDS; 1 case of ANLL exists oncogene c- myc and tumor suppressor gene p53 co-amplification, and found that amplification and MDS classification and clinical status of ANLL have a certain correlation.