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目的 研究一种新型黏膜佐剂 (一段寡核苷酸 ,序列为 :非甲基化的 5′ …嘌呤 嘌呤 CpG 嘧啶 嘧啶 … 3′(CpG ODN)是否可作为幽门螺杆菌 (Hp)疫苗的佐剂成分。 方法 C5 7BL/6小鼠经口灌胃给予Hp整菌超声粉碎物 (WCS) /CpG ODN或WCS/霍乱毒素 (CT) ,或经鼻给予WCS/CpG ODN ,并设立相应对照组。免疫程序及途径为每周 1次 ,共 4次 ,最后 1次免疫后 1周 ,以 5× 10 8Hp活菌攻击小鼠 ,攻击后 2周和 8周时 ,处死小鼠 ,鉴定Hp感染情况。同时收集小鼠血清、唾液、胃液 ,ELISA法检测血清中IgG、IgG1、IgG2a及IgA水平和唾液、胃液中IgA水平。 结果 以WCS作为抗原 ,不同佐剂及接种途径免疫小鼠 ,保护率分别为 :口服CT组 75 % (9/12 ) ,口服CpG ODN组 0 % (0 /10 ) ,滴鼻CpG ODN组 70 % (7/10 )。第 2和第 8周滴鼻CpG ODN组小鼠血清IgG2a水平显著高于未免疫的对照组(P <0 .0 5 )。结论 通过鼻道免疫CpG ODN是一很有前景的Hp疫苗佐剂成分。
Objective To study whether a novel mucosal adjuvant (a oligonucleotide sequence: unmethylated 5 ’purine CpG ODN) can be used as a vaccine against Helicobacter pylori (Hp) Agent composition. Methods C5 7BL / 6 mice were orally gavaged with either H. pylori (WCS) / CpG ODN or WCS / cholera toxin (CT), or nasally administered WCS / CpG ODN, and a corresponding control group Immunization procedures and routes were once a week for 4 times, 1 week after the last immunization, mice were challenged with 5 × 10 8 HP viable bacteria and mice were sacrificed at 2 weeks and 8 weeks after challenge to identify Hp infection The levels of IgG, IgG1, IgG2a and IgA in serum and the level of IgA in saliva and gastric juice were detected by ELISA.Results WCS was used as antigen, and different adjuvants and routes of vaccination were used to protect mice The rates were 75% (9/12) in the oral CT group, 0% (0/10) in the oral CpG ODN group and 70% (7/10) in the nasal CpG ODN group. The nasal CpG ODN The level of serum IgG2a in group mice was significantly higher than that in non-immunized group (P <0.05) .Conclusion CpG ODN is a very effective Hp vaccine adjuvant component views.