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目的 :为验证肿瘤坏死因子 (TNF)及其可溶性受体 (s TNFR)失衡导致复发性口疮发病的假说。方法 :采用双抗体夹心酶联免疫法检测组织标本中 TNFα、TNFβ及 s TNFR分泌水平 ,复发性口疮 (RAU)活动期患者 2 1例作为研究组 ,无 RAU病史的口腔粘膜外伤 2 3人作对照组。结果 :RAU病灶中 TNFα、TNFβ分泌水平明显高于对照组 ;RAU粘膜病灶的 s TNFR- 水平明显高于对照组 ,但 RAU病灶中口腔粘膜固有层单个核细胞分泌s TNFR- 并未升高。结论 :RAU患者的 TNFα分泌增多 ,但其粘膜固有层单个核细胞分泌 s TNFR- 的数量并未相应升高。RAU患者 TNF与 TNF天然抑制因子分泌失衡在其致病机制中占有重要意义。
OBJECTIVE: To test the hypothesis that the imbalance of tumor necrosis factor (TNF) and soluble receptor (s TNFR) causes recurrent aphthous ulcers. Methods: Tumor necrosis factor α (TNFα), tumor necrosis factor (TNF) and tumor necrosis factor-α (TNFR) secretion were detected by sandwich enzyme-linked immunosorbent assay. Twenty-one patients with recurrent aphthous ulcer (RAU) were enrolled as study group. Control group. Results: The levels of TNFα and TNFβ in RAU lesions were significantly higher than those in control group. The s TNFR level in RAU mucosal lesions was significantly higher than that in control group. However, TNFα secreted by mononuclear cells in RAU lesions did not increase in RAU lesions. CONCLUSIONS: TNFα secretion increased in patients with RAU, but the number of mononuclear cells secreting TNF-α in mucosa lamina propria did not increase correspondingly. The imbalance of secretion of TNF and TNF in RAU patients plays an important role in the pathogenesis of RAU.