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应用气相色谱-质谱/质谱法测定了茶叶中蒽醌残留量.前处理方法包括添加同位素内标蒽醌-D8,正己烷-丙酮(1:1,V/V)提取,弗罗里硅土柱净化,正己烷-乙醚(8:2,V/V)洗脱.采用HP-5MS色谱柱(30 m×0.25 mm×0.25μm)并使用程序升温过程对样品进行分离.选择反应监测模式(SRM),蒽醌两对离子进行定性、定量分析,内标法定量.蒽醌在0~200 mg/L范围内具有良好的线性关系,相关系数大于0.9990.在0.02,0.04和0.08 mg/kg 3个添加水平,绿茶、红茶、乌龙茶、普洱茶平均回收率范围为84.2%~98.1%,相对标准偏差小于9.7%,定量限为0.02 mg/kg.方法可用于茶叶样品中蒽醌残留的确证检测.“,”A method for the determination of anthraquinone in tea sample with GC-MS/MS has been introduced. The isotope internal standard ( anthraquinone-D8 ) was added into the sample. Sample was extracted by n-hexane:acetone (1:1, V/V) solution, cleaned-up with Florisil SPE columns, and then diluted with n-hexane: ethyl ether (8:2, V/V) solution. The samples were separated on HP-5MS capillary column (30 m × 0. 25 mm ( i. d. ), 0. 25 μm ( film thickness)) with temperature programming, and then detected by SRM mode using one precursor/two product ion transitions. The anthraquinone was quantified by the internal standard method. The results showed the method has good linearity in the range of 0~200 g/L with correlation coefficients above 0 . 9990 . The recoveries of green tea, black tea, pu 'er tea and oolong were between 84. 2% ~98. 1%, and the relative standard deviations (RSDs) were less than 9. 7% at the spiked levels of 0. 02, 0. 04 and 0. 08 mg/kg. The limit of quantitation was 0. 02 mg/kg. The method is simple, rapid, and accurate, and its performance can meet the requirements of the domestic and international legislation. The method could be applied to confirming residues of anthraquinone in tea samples.