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The effect of Mg~(2+) on the slow motion of H~+-ATPase in proteoliposome was studied. The ST-ESR spectra of H~+-ATPase labeled with maleimide and incorporated in liposomes at various Ma~(2+)concentrations with incubation method were measured. The relationship between the diagnostic param-eters L“/L, C’/C derived from ST-ESR spectra and Mg~(2+) concentrations or temperatures was com-pared with that of the order parameter S derived from 5NS-ESR spectra under the same conditions. Obtain-ed results clearly showed that the increase of Mg~(2+) concentration or the decrease of temperature maycause the increase of the rotational correlation time of H~+-ATPase in proteoliposome. And the rota-tional diffusion rate of H~+-ATPase in membrane lipid is related to the activation energy of the sur-rounding lipid. The change of Mg~(2+) concentration in proteoliposome leads to a change of activarionenergy and influence in turn the slow motion of H~+-ATPase in 1ipid.
The effect of Mg ~ (2+) on the slow motion of H ~ + -ATPase in proteoliposome was studied. The ST-ESR spectra of H ~ + -ATPase labeled with maleimide and incorporated in liposomes at various Ma ~ (2+) concentrations with incubation method were measured. The relationship between the diagnostic param-eters L ”/ L, C ’/ C derived from ST-ESR spectra and Mg ~ (2+) concentrations or temperatures was com-pared with that of the order parameter S derived from 5NS-ESR spectra under the same conditions. Obtain-ed results clearly showed that the increase of Mg ~ (2+) concentration or the decrease of temperature may cause the increase of the rotational correlation time of H ~ + -ATPase in And the rota-tional diffusion rate of H ~ + -ATPase in membrane lipid is related to the activation energy of the sur-rounding lipid. The change of Mg ~ (2+) concentration in proteoliposome leads to a change of activarionenergy and influence in turn the slow motion of H ~ + -ATPase in 1ipid.